HSF1 Polyclonal Antibody, Rockland™

Catalog No.	Quantity
50-199-2114	25 μg
50-199-2113	100 μg

Catalog No. 50-199-2114 Supplier Rockland Immunochemicals Supplier No. 600401GV3S

Description

Rabbit Polyclonal Antibody

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Anti-HSF1 is directed against human HSF1 at a n-terminus position. A BLAST analysis was used to suggest reactivity with this protein in mouse and bovine species based on 100% homology for the immunogen sequence.

All organisms respond to elevated temperatures and a variety of environmental stresses by rapid synthesis of heat shock RNAs and proteins. The regulation of heat shock gene transcription is mediated by the transcriptional activator, heat shock factor (HSF), which binds to heat shock response elements (HSEs). These HSEs are found as three repeats of a 5-nucleotide {nGAAn} module, arranged in alternating orientation and present upstream of all heat shock genes. The HSEs are highly conserved among species yet HSF purified from yeast, Drosophila and human have different molecular weights and the proteins do not show significant immunological cross reaction. Two HSFs have been identified in human cells, HSF1 and HSF2, which bind to the same HSEs and have 38% sequence identity. These factors are activated by distinct stimuli, HSF1 is responsive to classical stress signals such as heat, heavy metals and oxidative reagents, whereas HSF2 is activated during hemin-mediated differentiation of human erythroleukemia cells. HSF1 exists constitutively in the cytoplasm and the nucleus of unstressed cells as a monomer which lacks DNA binding activity. Through an unknown signal generated during stress, HSF1 becomes activated to a nuclear localized, trimeric state which binds to DNA. The phosphorylation of HSF1 is necessary for maximal transcription of heat shock genes.

Specifications

• Antigen: HSF1
• Applications: ELISA, Western Blot
• Classification: Polyclonal
• Concentration: 1 mg/mL
• Conjugate: Unconjugated
• Formulation: 0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2
• Gene: Hsf1
• Gene Accession No.: Q00613
• Gene Alias: AA960185; BOS_13649; heat shock factor 1; Heat shock factor protein 1; Heat shock transcription factor 1; heat shock transcription factor 1 alpha isoform; heat shock transcription factor 1 beta isoform; heat shock transcription factor 1 gammaalpha isoform; heat shock transcription factor 1 gammabeta isoform; HSF 1; Hsf1; Hsf1alpha; Hsf1beta; Hsf1gammaalpha; Hsf1gammabeta; HSTF 1; HSTF1
• Gene Symbols: Hsf1
• Host Species: Rabbit
• Quantity: 25 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 3297
• Target Species: Human
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Product Type: Antibody
• Form: Liquid