Invitrogen™ HSP27 Monoclonal Antibody (8A7), FITC

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA545122

Catalog No. PIMA545122

Description

A 1:5000 dilution of MA5-45122 was sufficient for detection of HSP27 in 20 μg of HeLa cell lysate by ECL immunoblot analysis. Detects approximately 25kDa or approximately 27kDa. Recognizes HSP25 and HSP27, cross reacts with alpha B crystallin.

In response to adverse changes in their environment, cells from many organisms increase the expression of a class of proteins referred to as heat shock or stress proteins. HSPB1 (heat shock protein beta-1 or HSP27) is a small heat shock protein which functions as a molecular chaperone that maintains denatured proteins in a folding-competent state. It plays a role in stress resistance and actin organization. Through its molecular chaperone activity, HSP27 regulates numerous biological processes including the phosphorylation and the axonal transport of neurofilament proteins. Mutations in the gene can result in Charcot-Marie-Tooth disease 2F and Neuronopathy distal hereditary motor 2B.

Specifications

• Antigen: HSP27
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS, 9.1mM sodium bicarbonate with 640.91mM DMSO, 136.36mM ethanolamine and no preservative
• Gene Accession No.: P04792, P14602, P42929, P42930, Q3T149
• Gene Symbols: HSPB1
• Immunogen: HSP27 peptide.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat, Canine, Bovine, Sheep, Hamster, Guinea Pig
• Product Type: Antibody
• Isotype: IgG1 κ

• Applications: Flow Cytometry, Immunohistochemistry (Paraffin), Immunohistochemistry (PFA fixed), Immunoprecipitation, Western Blot, Immunocytochemistry
• Clone: 8A7
• Conjugate: FITC
• Gene: HSPB1
• Gene Alias: 27kDa; 28 kDa heat shock protein; CMT2F; DKFZp586P1322; epididymis secretory protein Li 102; estrogen-regulated 24 kDa protein; Growth-related 25 kDa protein; heat shock 25 kDa protein; Heat shock 27 kDa protein; heat shock 27kD protein 1; heat shock 27kDa protein 1; Heat shock protein; heat shock protein 1; heat shock protein 27 kDa beta-1; heat shock protein B1; heat shock protein beta-1; heat shock protein family B (small) member 1; heat shock protein, 25 kDa; heat-shock protein; HEL-S-102; HMN2B; HS; HS.76067; HSP; HSP 25; HSP 27; Hsp25; HSP27; HSP28; HSPB1; p25; Phospho-HSP 27; SRP27; Stress-responsive protein 27; truncated hsp25
• Host Species: Mouse
• Purification Method: Protein G
• Regulatory Status: RUO
• Gene ID (Entrez): 100724197, 100763481, 101115754, 15507, 24471, 3315, 403979, 516099
• Content And Storage: 4°C, store in dark
• Form: Liquid

Safety and Handling

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