Invitrogen™ HSP40 Monoclonal Antibody (3B9.E6)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA527685

Catalog No. PIMA527685

Description

Immunohistochemistry samples may be prepared using cold 100% methanol at -20C for fixation. 0.5 μg/mL of MA5-27685 was sufficient for detection of HSP40 (HDJ1) in 15 μg of HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. Detects approximately 40kDa. Does not cross-react with HDJ2 or YDJ1.

TCF-9 (transcription factor 9), also known as C2orf3 (chromosome 2 open reading frame 3), GCF (GC-rich sequence DNA-binding factor) or DNABF, is a 781 amino acid nuclear protein that belongs to the GCF family. Widely expressed, GCF binds the GC-rich sequences of beta-Actin, EGFR and calcium-dependent protease (CANP) promoters. GCF contains multiple phosphoserine and phosphothreonine residues, and two GCF isoforms are produced due to alternative splicing events. GCF is considered a candidate for susceptibility to dyslexia (DYX3) as both genes reside in close proximity on human chromosome 2. Chromosome 2 is the second largest human chromosome and consists of 237 million bases, encodes over 1,400 genes and makes up approximately 8% of the human genome.

Specifications

• Antigen: HSP40
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with 50% glycerol and 0.09% sodium azide; pH 7.2
• Gene Accession No.: P25685, Q9QYJ3
• Gene Symbols: DNAJB1
• Immunogen: Recombinant Protein human HSP40 (Hdj1).
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG1

• Applications: ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot, Immunocytochemistry
• Clone: 3B9.E6
• Conjugate: Unconjugated
• Gene: DNAJB1
• Gene Alias: 0610007I11Rik; DjB1; DnaJ (Hsp40) homolog, subfamily B, member 1; DnaJ heat shock protein family (Hsp40) member B1; dnaJ homolog subfamily B member 1; DnaJ protein homolog 1; DNAJ1; Dnajb1; HDJ1; hDj-1; Heat shock 40 kDa protein 1; Heat shock protein; HSP; HSP 40; HSP40; HSPF1; human DnaJ protein 1; NDAJB1; radial spoke 16 homolog B; RSPH16B; Sis1
• Host Species: Mouse
• Purification Method: Protein G
• Regulatory Status: RUO
• Gene ID (Entrez): 3337, 361384, 81489
• Content And Storage: -20°C
• Form: Liquid