Learn More
Invitrogen™ Human HSPB1 (HSP27) ELISA Kit

Description
The Human HSPB1 (HSP27) ELISA quantitates Hu HSP27 in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu HSP27. Principle of the method The Human HSP27BTC solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Specifications
Specifications
Accession Number | P04792 |
Assay Range | 0.205 to 50 ng/mL |
Assay Sensitivity | 150 pg/mL |
Calibration Range | 0.15 ng/mL to 50 ng/mL |
Assay | Solid-phase sandwich ELISA |
Conjugate | HRP |
Product Type | ELISA |
Protein Family | Growth Factors & Receptors |
Sample Type | Plasma, Serum, Supernatant |
Specificity | This ELISA antibody pair detect human HSP27. Other species not determined. |
Show More |
The Fisher Scientific Encompass Program offers items which are not part of our distribution portfolio. These products typically do not have pictures or detailed descriptions. However, we are committed to improving your shopping experience. Please use the form below to provide feedback related to the content on this product.