Invitrogen™ HuC/HuD Monoclonal Antibody (16A11)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ A21271

Catalog No. A21271

Description

This antibody recognizes the Elav family members HuC, HuD and Hel-N1 neuronal proteins. It does not recognize HuR, another Elav family member that is present in all proliferating cells. The antibody has been shown to specifically label neuronal cells in zebrafish, chick, canaries, and humans, and is likely to label neuronal cells in most vertebrate species. Labeling is visible early in development, at about the time that the neurons leave the mitotic cycle. Storage and reconstitution: Upon receipt, lyophilized antibodies should be stored desiccated at -20ºC or lower. When properly stored, these products are stable for at least one year. To prepare stock solution, reconstitute the antibody in 0.5-1 mL PBS, pH 7.4, containing 1% BSA. Store the solution for up to two weeks at 4ºC with the addition of 2mM sodium azide. For longer storage, divide solutions into single-use aliquots and freeze at -20ºC, avoidig freeze/thaw cycles. For best results in immunohistochemistry, antigen retrieval is required. Use 50mM Tris, pH 8.0, for 30 minutes. A-21271 was successfully used to detect HuC/HuD in neurons differentiated from H9 ESC derived NSCs.

Anti-Hu antibodies were originally isolated from a patient with paraneoplastic encephalomyelitis, a rare condition of selective neural tissue injury associated with small cell lung carcinoma. The antibodies bind specifically to antigens present exclusively in neuronal cells and are thus useful as markers of neuronal cells in tissue. The Hu antigen is an RNA-binding protein of the embryonic lethal abnormal visual (Elav) family.

Specifications

• Antigen: HuC/HuD
• Classification: Monoclonal
• Conjugate: Unconjugated
• Gene: ELAVL4
• Gene Alias: ELAV (embryonic lethal, abnormal vision, Drosophila)-like 3 (Hu antigen C); ELAV (embryonic lethal, abnormal vision, Drosophila)-like 4 (Hu antigen D); ELAV like neuron-specific RNA binding protein 3; ELAV like neuron-specific RNA binding protein 4; ELAV like RNA binding protein 4; elavC; ELAVL3; elavl3 protein; elavl4; ELAV-like protein 3; ELAV-like protein 4; elav-related C; elav-related D; elrc; elrd; embryonic lethal, abnormal vision-related C; embryonic lethal, abnormal vision-related D; etID309927.3; Hu antigen C; Hu antigen D; hu-antigen C; Hu-antigen D; HuC; HUCL; hud; id:ibd1248; Paraneoplastic cerebellar degeneration-associated antigen; paraneoplastic encephalomyelitis antigen HuD; Paraneoplastic limbic encephalitis antigen 21; PLE21; PNEM; protein elrD; ribonucleoprotein; RNA-binding protein HuD; wu:fb77b03; wu:fc24h01; zHuC
• Host Species: Mouse
• Purification Method: purified
• Regulatory Status: RUO
• Gene ID (Entrez): 1995, 1996, 30732, 30737, 395634
• Content And Storage: -20°C
• Form: Lyophilized

• Applications: Immunohistochemistry (Frozen), Western Blot, Immunocytochemistry
• Clone: 16A11
• Formulation: HEPES buffered saline with no preservative
• Gene Accession No.: P26378, Q14576
• Gene Symbols: ELAVL3, ELAVL4
• Immunogen: Human HuC/HuD neuronal protein.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Chicken, Avian, Zebrafish
• Product Type: Antibody
• Isotype: IgG2b κ