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R&D Systems™ Human Active MMP-9 Fluorokine E Kit

Catalog No. F9M00
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Description

Contains Mercury, Dispose According to Local, State or Federal Laws.

The Fluorokine™ E Human Active MMP-9 Florescent Assay combines the specificity of a monoclonal antibody that captures all three MMP-9 forms (92, 82, and 65 kDa) but not other MMPs and the sensitivity of fluorescence. The assay is designed to measure the levels of endogenous human active MMP-9 in cell culture supernates, serum, plasma, and urine and the MMP-9 in these samples that can be activated by APMA during the assay procedure. The determined MMP-9 activity may reflect the balance of MMP-9 and its inhibitor TIMPs. Therefore, it is recommended that the levels of TIMPs be determined in order to interpret the results properly. For example, TIMP-1 inhibits the activity of MMP-9 through binding of the N-terminal domain of the TIMP-1 to the active site of MMP-9. In addition, TIMP-1 also binds to pro-MMP-9 through the interaction between the C-terminal domains of both proteins .

Specifications

Content And Storage Store the unopened product at 2°C to 8°C. Do not use past expiration date.
Gene ID (Entrez) 4318
Gene Symbol MMP9
Quantity 1 Kit
Synonym 92 kDa gelatinase, 92 kDa type IV collagenase, CLG4B, EC 3.4.24, EC 3.4.24.35, Gelatinase B, GELB, macrophage gelatinase, MANDP2, matrix metallopeptidase 9, matrix metalloproteinase 9, matrix metalloproteinase-9, MMP9, MMP-9, type V collagenase
Target MMP-9
Assay Range 0.25 to 16 pg/mL
Assay Sensitivity 0.01 pg/mL
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