Invitrogen™ Human ADAMTS-13 ELISA Kit

Description

ELISA

Human ADAMTS-13 quantitates human ADAMTS-13 in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant human ADAMTS-13.

ADAMTS13 is a member of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motif) protein family. Members of the family share several distinct protein modules, including a propeptide region, a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. Individual members of this family differ in the number of C-terminal TS motifs, and some have unique C-terminal domains. The enzyme is the von Willebrand Factor (vWF)-cleaving protease, which is responsible for cleaving at the site of Tyr842-Met843 of the vWF molecule. A deficiency of this enzyme is associated with thrombotic thrombocytopenic purpura.

Specifications

• Accession Number: a disintegrin and metalloproteinase; A disintegrin and metalloproteinase with thrombospondin motifs 13; A disintegrin and metalloproteinase with thrombospondin motifs 13-like; a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 13; a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 13; ADAM; ADAM metallopeptidase with thrombospondin type 1 motif 13; ADAM metallopeptidase with thrombospondin type 1 motif, 13; ADAMs; ADAM-TS 13; Adamts13; ADAM-TS13; ADAMTS-13; C9orf8; Gm710; LOC102554393; metalloendopeptidases; UNQ6102/PRO20085; von Willebrand factor-cleaving protease; vWF-cleaving protease; VWFCP; vWF-CP; vWF-CP mRNA for von Willebrand factor-cleaving
• Assay Range: 7.5 to 1800 ng/mL
• Assay Sensitivity: 7.5 ng/mL
• Conjugate: HRP
• Product Type: ELISA
• Sample Type: Plasma, Serum, Supernatant
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 11093
• Gene Symbol: ADAMTS13
• Interassay CV: <12%
• Intraassay CV: <10
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: HRP
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
• Storage Requirements: 2°C to 8°C
• Target: ADAMTS13
• Test Time: 1 hrs 20 mins
• Total Assay Time: 4 hrs 45 mins