Abnova™ Human AKT1 (NM_005163, 106 a.a. - 480 a.a) Partial Recombinant Protein with GST-His tag expressed in Sf9 cells

Description

Used for Func, SDS-PAGE

The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq]

Specifications

• Accession Number: NM_005163
• Concentration: 0.434 ug/uL
• For Use With (Application): Functional Study, SDS-PAGE
• Formulation: Liquid
• Gene ID (Entrez): 207
• Molecular Weight (g/mol): 77.832kDa
• Name: AKT1 (Human) Recombinant Protein
• Preparation Method: Insect cell (Sf9) expression system
• Purification Method: One-step affinity purification using GSH-agarose
• Quality Control Testing: 2 ug/lane SDS-PAGE Stained with Coomassie Blue
• Quantity: 100 μg
• Storage Requirements: Store at -80°C. Aliquot to avoid repeated freezing and thawing
• Regulatory Status: RUO
• Gene Alias: AKT/MGC99656/PKB/PKB-ALPHA/PRKBA/RAC/RAC-ALPHA
• Common Name: AKT1
• Gene Symbol: AKT1
• Biological Activity: 328pmol/μg x min
• Species: Baculovirus
• Recombinant: Recombinant
• Protein Tag: GST-His
• Expression System: Insect cell (Sf9) expression system
• Form: Liquid