R&D Systems™ Human Angiopoietin-like 6, Carrier-Free Recombinant Protein

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility.

Measured by its binding ability in a functional ELISA. When Recombinant Human Angiopoietin-like Protein 6/ANGPTL6 (Catalog # 8466-AN/CF) is immobilized at 2 μg/mL (100 μL/well), Recombinant Human LILRB2/CD85d/ILT4 Fc Chimera (Catalog # 2078-T4 ) binds with an ED50 of 30.0-360 ng/mL.

Specifications

• Accession Number: Q8NI99
• For Use With (Application): Bioactivity
• Gene ID (Entrez): 83854
• Molecular Weight (g/mol): Observed M.W.: 58-69kDa and 31-41kDa, reducing conditions, Theoretical M.W.: 51kDa
• Quantity: 25 μg
• Source: Chinese Hamster Ovary cell line,CHO-derived human Angiopoietin-like Protein 6/ANGPTL6 protein Ala20-Leu470,with an N-terminal c-Myc tag
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70° C as supplied. 1 month, 2 to 8° C under sterile conditions after reconstitution. 3 months, -20 to -70° C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.10 EU per 1μg of the protein by the LAL method.
• Gene Alias: AGF, AGFAngiopoietin-related protein 5, Angiopoietin like Protein 6, angiopoietin-like 6, Angiopoietin-like protein 6, Angiopoietin-related growth factor, ANGPTL6, ARP5, ARP5angiopoietin-related protein 6
• Common Name: Angiopoietin-like Protein 6/ANGPTL6
• Gene Symbol: ANGPTL6
• Biological Activity: Measured by its ability to promote the expansion of E16 rat liver mononuclear cells in vitro, in the presence of Recombinant Mouse SCF/c-kit Ligand, Recombinant Mouse Thrombopoietin/Tpo, and Recombinant Mouse Flt-3 Ligand. The ED50 for this effect is typically 8-40ng/mL in the presence of a cross-linking Mouse Anti-Human c-Myc Monoclonal Antibody.
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Expression System: Chinese Hamster Ovary cell line, CHO-derived Ala20-Leu470, with an N-terminal c-Myc tag
• Purity or Quality Grade: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining