R&D Systems™ Recombinant Human ASIP/Agouti Protein

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility.

The Recombinant Human ASIP/Agouti Protein is derived from HEK293. The Recombinant Human ASIP/Agouti Protein has been validated for the following applications: Bioactivity.

Specifications

• Accession Number: P42127
• For Use With (Application): Bioactivity
• Gene ID (Entrez): 434
• Molecular Weight (g/mol): Observed M.W.: 18-23kDa, reducing conditions, Theoretical M.W.: 12kDa
• Quantity: 50 μg
• Source: Human embryonic kidney cell,HEK293-derived human ASIP protein His23-Cys132
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70° C as supplied. 1 month, 2 to 8° C under sterile conditions after reconstitution. 3 months, -20 to -70° C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.10 EU per 1μg of the protein by the LAL method.
• Gene Alias: agouti (mouse)-signaling protein, agouti signaling protein, agouti signaling protein, nonagouti homolog, agouti signaling protein, nonagouti homolog (mouse), Agouti switch protein, agouti-signaling protein, AGSW, AGTI, AGTIL, AGTILMGC126092, ASPAGSW, MGC126093, nonagouti homolog, SHEP9
• Common Name: ASIP
• Gene Symbol: ASIP
• Biological Activity: Measured by its ability to inhibit alpha-MSH induced eumelanin production in B16F1 mouse melanoma cells. The ED50 for this effect is typically 0.8-4μg/mL.
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Expression System: Human embryonic kidney cell, HEK293-derived His23-Cys132
• Purity or Quality Grade: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining