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Human B7-1/CD80 Antibody, R&D Systems™
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Catalog No. MAB140SP
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Catalog No. Quantity
MAB140SP 25 μg
MAB140100 100 μg
MAB140500 500 μg
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Catalog No. MAB140SP Supplier R&D Systems Supplier No. MAB140SP
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Description

Mouse Monoclonal Antibody has been used in 9 publications

B7-1/CD80 Monoclonal specifically detects B7-1/CD80 in Human samples. It is validated for Flow Cytometry, Immunohistochemistry, Neutralization, CyTOF-ready, Dual RNAscope ISH-IHC, ELISA Capture (Matched Antibody Pair).

Specifications

Antigen B7-1/CD80
Applications Flow Cytometry, Immunohistochemistry, Neutralization, CyTOF, In Situ Hybridization (ISH), ELISA
Classification Monoclonal
Clone 37711
Concentration LYOPH
Conjugate Unconjugated
Dilution Flow Cytometry 0.25 ug/10^6 cells, Immunohistochemistry 5-25 ug/mL, Neutralization 0.2-1 ug/mL, CyTOF-ready, Dual RNAscope ISH-IHC 5-25 ug/mL, ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS. with No Preservative
Gene Accession No. P33681
Gene Alias Activation B7-1 antigen, B7, B71, BB1, CD28LG1, CD28LGB7-1 antigen), CD80, CD80 antigen, CD80 molecule, costimulatory factor CD80, costimulatory molecule variant IgV-CD80, CTLA-4 counter-receptor B7.1, T-lymphocyte activation antigen CD80
Gene Symbols CD80
Host Species Mouse
Immunogen S. frugiperda insect ovarian cell line Sf 21-derived recombinant human B7-1/CD80 Val35-Asn242 (predicted) Accession # P33681
Purification Method Protein A or G purified from hybridoma culture supernatant
Quantity 25 μg
Regulatory Status RUO
Primary or Secondary Primary
Gene ID (Entrez) 941
Test Specificity Detects human B7-1/CD80 in ELISAs. In sandwich immunoassays, no cross-reactivity or interference with recombinant human (rh) B7-2, recombinant mouse (rm) B7-1, rmB7-2, rhB7-H1, rhB7-H2, rhB7-H3 or rmPD-L2 is observed.
Reconstitution Reconstitute at 0.5 mg/mL in sterile PBS.
Target Species Human
Content And Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Form Purified
Isotype IgG1
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