Human B7-H3 Antibody, R&D Systems™

Goat Polyclonal Antibody has been used in 16 publications

Supplier:  R&D Systems AF1027SP

Catalog No. AF1027SP

Description

B7-H3/CD276 Polyclonal specifically detects B7-H3/CD276 in Human samples. It is validated for Western Blot, Simple Western, Flow Cytometry, Immunohistochemistry, CyTOF-ready, Knockout Validated.

Specifications

• Antigen: B7-H3/CD276
• Classification: Polyclonal
• Conjugate: Unconjugated
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS. with No Preservative
• Gene Alias: B7H3, B7H34Ig-B7-H3, B7-H3B7 homolog 3, CD276, CD276 antigen, CD276 molecule, Costimulatory molecule
• Host Species: Goat
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Gene ID (Entrez): 80381
• Reconstitution: Reconstitute at 0.2 mg/mL in sterile PBS.
• Content And Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

• Applications: Western Blot, Flow Cytometry, Immunohistochemistry, CyTOF, KnockDown
• Concentration: LYOPH
• Dilution: Western Blot 1 ug/mL, Simple Western 10 ug/mL, Flow Cytometry 0.25 ug/10^6 cells, Immunohistochemistry 5-15 ug/mL, CyTOF-ready, Knockout Validated
• Gene Accession No.: NP_079516
• Gene Symbols: CD276
• Immunogen: Mouse myeloma cell line NS0-derived recombinant human B7-H3 Leu29-Pro245 Accession # NP_079516
• Quantity: 25 μg
• Primary or Secondary: Primary
• Test Specificity: Detects human B7-H3 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human (rh) B7-H2 is observed, and less than 1% cross-reactivity with rhB7-1, rhB7-2, rhB7-H1, and rhPD-L2 is observed.
• Target Species: Human
• Isotype: IgG

For Research Use Only