Invitrogen™ BCL-2 Human ELISA Kit

For the quantitative detection of human Bcl-2.

Supplier:  Invitrogen™ BMS2443

Catalog No. 50-181-49

Includes: Aluminum pouch(es) with a Microwell Plate coated with monoclonal antibody to human Bcl-2
Biotin-Conjugate anti-human Bcl-2 monoclonal antibody
Streptavidin-HRP
Human Bcl-2 Standard lyophilized, 64ng/mL upon reconstitution
Sample Diluent
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Lysis Buffer 10x
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Adhesive Films

Description

The Human Bcl-2 ELISA quantitates Hu Bcl-2 in human serum, plasma, or cell lysates. The assay will exclusively recognize both natural and recombinant Hu Bcl-2. Principle of the method The Human Bcl-2 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

BCL-2 is a key regulator of apoptosis that functions to either inhibit or promote cell death. The BCL-2 family members are also characterized by dimerizing to further modulate apoptosis. Bag1, for example, has been found to form a heterodimer with BCL-2 resulting in the enhancement of the anti-apoptotic effect of BCL-2. Bax and Bak have been shown to play a critical role in cytochrome c release from mitochondria and thus initiate apoptosis. Bax exerts a pro-apoptotic rather than an anti-apoptotic effect on cells. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. In most follicular lymphomas, neoplastic germinal centers express high levels of BCL-2 alpha protein, whereas the normal or hyperplastic germinal centers are negative. Two transcript variants of BCL-2, produced by alternate splicing, differ in their C-terminal ends. The overexpression of BCL-2 has been linked to human cancers such as B-cell lymphoma and prostate cancer.

Specifications

• Accession Number: P10415
• Assay Sensitivity: <0.5 ng/mL
• Product Type: ELISA Kit
• Target Species: Human
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: Bcl-2,PPP1R50
• Intraassay CV: 8.6%
• Label or Dye: HRP
• Regulatory Status: RUO
• Storage Requirements: 2°C to 8°C
• Target: Human
• Total Assay Time: 3 hr. 10 min.

• Assay Range: 0.50-32.0 ng/mL
• Conjugate: Biotin
• Sample Type: Cell Lysates, Plasma, Serum
• For Use With (Application): ELISA
• Gene ID (Entrez): 596
• Interassay CV: 12%
• Kit Contents: Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Lysis Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Quantity: 96 Tests
• Sample Volume: Cell Lysate,20 μL; Plasma, 20 μL; Serum, 20 μL
• Synonym: Apoptosis regulator Bcl-2
• Test Time: 1 hr. 20 min.

For Research Use Only