Gibco™ Human BMP-4 Recombinant Protein, PeproTech®

Catalog No.	Quantity
50-162-4291	100 μg
12005ET10UG	10 μg
12-005-ET01M	2 x 500 μg
12005ET250	250 μg
12005ET2UG	2 μg
12005ET500	500 μg
12005ET50UG	50 μg

Catalog No. 50-162-4291 Supplier Gibco™ Supplier No. 12005ET100UG

Description

Recombinant Protein

120-05ET-1MG will be provided as 2 x 500 μg (120-05ET-500UG). PeproTech's E.coli-derived BMP-4 is a fully active homodimeric protein consisting of two 106 amino acid subunits, which correspond to amino acids 303-408 of the full length BMP-4 precursor. The calculated molecular weight of Recombinant Human BMP-4 (E.coli-derived) is 23.9 kDa. This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

The protein encoded by this gene is a member of the bone morphogenetic protein family which is part of the transforming growth factor-beta superfamily. The superfamily includes large families of growth and differentiation factors. Bone morphogenetic proteins were originally identified by an ability of demineralized bone extract to induce endochondral osteogenesis in vivo in an extraskeletal site. This particular family member plays an important role in the onset of endochondral bone formation in humans, and a reduction in expression has been associated with a variety of bone diseases, including the heritable disorder Fibrodysplasia Ossificans Progressiva. Alternative splicing in the 5' untranslated region of this gene has been described and three variants are described, all encoding an identical protein.

Specifications

• Accession Number: P12644
• For Use With (Application): Control, Western Blot Control, Bioactivity
• Formulation: Protein with no preservative
• Gene ID (Entrez): 652
• Molecular Weight (g/mol): 23.9 kDa
• Name: Human BMP-4
• Purification Method: Purified
• Quantity: 100 μg
• Source: E. coli
• Storage Requirements: -20°C
• Regulatory Status: RUO
• Endotoxin Concentration: <1 EU/μg
• Gene Alias: BMP; BMP2B; BMP-2B; Bmp2b1; Bmp2b-1; Bmp4; Bmp-4; BOMPR4A; Bone morphogenetic protein; Bone morphogenetic protein 2B; bone morphogenetic protein 4; bone morphogenetic protein 4 preproprotein; DVR4; Dvr-4; MCOPS6; OFC11; ZYME
• Common Name: BMP4
• Gene Symbol: BMP4
• Biological Activity: Determined by its ability to induce alkaline phosphatase production by ATDC-5 cells. The expected ED50 for this effect is 5-10 ng/ml
• Product Type: Protein
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Sequence: KKNKNCRRHS LYVDFSDVGW NDWIVAPPGY QAFYCHGDCP FPLADHLNST NHAIVQTLVN SVNSSIPKAC CVPTELSAIS MLYLDEYDKV VLKNYQEMVV EGCGCR
• Content And Storage: -20°C
• Activity: Determined by its ability to induce alkaline phosphatase production by ATDC-5 cells. The expected ED50 for this effect is 5-10 ng/ml.
• Expression System: E. coli
• Form: Lyophilized
• Purity or Quality Grade: ≥ 98% by SDS-PAGE gel and HPLC analyses.