Gibco™ Human C1 Inhibitor Recombinant Protein, PeproTech®

Description

Recombinant Protein

Recombinant Human C1 Inhibitor is a highly glycosylated glycoprotein containing 478 amino acid residues (52.8kDa), corresponding to amino acids 23-500 of the C1 inhibitor precursor, and is fully functional in its ability to inhibit the C1 complex. Glycosylated C1 Inhibitor migrates at an apparent molecular weight of approximately 80-90 kDa by SDS PAGE analysis under reducing conditions. This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

C1-inhibitor (C1INH) functions as a regulator of the activation of the classical complement pathway and of the contact activation system of kinin generation and coagulation. Its primary biologically relevant target proteinases are C1r, C1s, coagulation factors XIa and XIIa, and plasma kallikrein. C1-inhibitor consists of one polypeptide chain and has a molecular mass of ∽104 kDa. Serum concentration has been determined to 180 μg/mL.

Specifications

• Accession Number: P05155
• For Use With (Application): Functional Assay
• Formulation: protein with no preservative
• Gene ID (Entrez): 710
• Molecular Weight (g/mol): 52.8 kDa
• Name: Human C1 Inhibitor
• Quantity: 1 mg
• Source: CHO cells
• Regulatory Status: RUO
• Endotoxin Concentration: <1 EU/ μg
• Gene Alias: C1 esterase inhibitor; C1 Inh; C1 inhibitor; C1IN; C1Inh; C1-inhibiting factor; C1nh; complement component 1 inhibitor; complement component 1 inhibitor (angioedema, hereditary); esterase inhibitor; factor XIIa inhibitor; HAE1; HAE2; IC1; Plasma protease C1 inhibitor; serine (or cysteine) peptidase inhibitor, clade G, member 1; serine (or cysteine) proteinase inhibitor, clade G (C1 inhibitor), member 1; serine (or cysteine) proteinase inhibitor, clade G (C1 inhibitor), member 1, (angioedema, hereditary); serine (or cysteine) proteinase inhibitor, clade G, member 1; serine/cysteine proteinase inhibitor clade G member 1; serpin family G member 1; serpin G1; serpin peptidase inhibitor clade G member 1; serpin peptidase inhibitor, clade G (C1 inhibitor), member 1; serpin peptidase inhibitor, clade G (C1 inhibitor), member 1, (angioedema, hereditary); serpin peptidase inhibitor, clade G, member 1; SERPING1; XIIaINH
• Common Name: C1 inhibitor
• Gene Symbol: SERPING1
• Biological Activity: Measured by its ability to inhibit recombinant human complement component C1a cleavage of a colorimetric peptide substrate, N Carbobenzyloxy-Lys-ThioBenzyl ester (Z-K-SBzl). The expected IC50 is ≤ 2.6 nM.
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Sequence: NPNATSSSSQ DPESLQDRGE GKVATTVISK MLFVEPILEV SSLPTTNSTT NSATKITANT TDEPTTQPTT EPTTQPTIQP TQPTTQLPTD SPTQPTTGSF CPGPVTLCSD LESHSTEAVL GDALVDFSLK LYHAFSAMKK VETNMAFSPF SIASLLTQVL LGAGENTKTN LESILSYPKD FTCVHQALKG FTTKGVTSVS QIFHSPDLAI RDTFVNASRT LYSSSPRVLS NNSDANLELI NTWVAKNTNN KISRLLDSLP SDTRLVLLNA IYLSAKWKTT FDPKKTRMEP FHFKNSVIKV PMMNSKKYPV AHFIDQTLKA KVGQLQLSHN LSLVILVPQN LKHRLEDMEQ ALSPSVFKAI MEKLEMSKFQ PTLLTLPRIK VTTSQDMLSI MEKLEFFDFS YDLNLCGLTE DPDLQVSAMQ HQTVLELTET GVEAAAASAI SVARTLLVFE VQQPFLFVLW DQQHKFPVFM GRVYDPRA
• Content And Storage: -20°C
• Expression System: CHO cells
• Form: Lyophilized
• Purity or Quality Grade: ≥ 95% by SDS-PAGE gel and HPLC analyses.