Invitrogen™ Human Carboxypeptidase B2/CPB2 ELISA Kit

Description

ELISA

Human Carboxypeptidase B2/CPB2 quantitates human Carboxypeptidase B2/CPB2 in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant human Carboxypeptidase B2/CPB2.

Carboxypeptidases are enzymes that hydrolyze C-terminal peptide bonds. The carboxypeptidase family includes metallo-, serine, and cysteine carboxypeptidases. According to their substrate specificity, these enzymes are referred to as carboxypeptidase A (cleaving aliphatic residues) or carboxypeptidase B (cleaving basic amino residues). CPB2 is activated by trypsin and acts on carboxypeptidase B substrates. After thrombin activation, the mature protein downregulates fibrinolysis. Polymorphisms have been described for its gene and its promoter region.

Specifications

• Accession Number: 1110032P04Rik; 4930405E17Rik; AI255929; carboxypeptidase B2; carboxypeptidase B2 (plasma); carboxypeptidase B2 (plasma, carboxypeptidase U); carboxypeptidase B-like protein; carboxypeptidase R; Carboxypeptidase U; Cpb2; CPR; CPU; PCPB; Plasma carboxypeptidase B; Tafi; thrombin-activable fibrinolysis inhibitor; thrombin-activatable fibrinolysis inhibitor
• Assay Range: 0.082 to 20 ng/mL
• Assay Sensitivity: 0.082 ng/mL
• Conjugate: HRP
• Product Type: ELISA
• Sample Type: Plasma, Serum, Supernatant
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 1361
• Gene Symbol: CPB2
• Interassay CV: <12%
• Intraassay CV: <10
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: HRP
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
• Storage Requirements: 2°C to 8°C
• Target: Carboxypeptidase B2
• Test Time: 1 hrs 20 mins
• Total Assay Time: 4 hrs 45 mins