Invitrogen™ Human CD27 Ligand/TNFSF7 ELISA Kit

Description

ELISA

Human CD27 Ligand/TNFSF7 quantitates human CD27 Ligand/TNFSF7 in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant human CD27 Ligand/TNFSF7.

The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for TNFRSF27/CD27. It is a surface antigen on activated, but not on resting, T and B lymphocytes. It induces proliferation of costimulated T cells, enhances the generation of cytolytic T cells, and contributes to T cell activation. This cytokine is also reported to play a role in regulating B-cell activation, cytotoxic function of natural killer cells, and immunoglobulin synthesis.

Specifications

• Accession Number: CD27 ligand; Cd27l; CD27-L; CD27LG; CD70; CD70 antigen; Cd70 molecule; Ki-24 antigen; surface antigen CD70; Tnfsf7; TNLG8A; tumor necrosis factor (ligand) superfamily, member 7; tumor necrosis factor ligand 8 A; tumor necrosis factor ligand superfamily member 7
• Assay Range: 0.3 to 70 ng/mL
• Assay Sensitivity: 0.3 ng/mL
• Conjugate: HRP
• Product Type: ELISA
• Sample Type: Plasma, Serum, Supernatant
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 970
• Gene Symbol: Cd70
• Interassay CV: <12%
• Intraassay CV: <10
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: HRP
• Quantity: 5 x 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
• Storage Requirements: 2°C to 8°C
• Target: CD70
• Test Time: 1 hrs 20 mins
• Total Assay Time: 4 hrs 45 mins

Safety and Handling

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