Gibco™ Human CTGF (CCN2) Recombinant Protein

Description

Recombinant Protein

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile, distilled water, to a concentration of 0.1-1.0 mg/mL. Do not vortex. This solution can be stored at 2°C to 8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein, such as 0.1% BSA and store in working aliquots at -20°C to -80°C. Storage: The lyophilized protein is stable at room temperature for 1 month, but should be kept at -20°C for long term storage, preferably desiccated. Working aliquots stored with a carrier protein are stable for at least 10 months at -20°C to -80°C. Avoid repeated freeze/thaw cycles.

Connective Tissue Growth Factor (CTGF), also called CCN2, is a member of the CCN family. CTGF is a secreted protein produced by umbilical veins and vascular endothelial cells. CTGF possesses an Insulin-like Growth Factor (IGF)-binding domain, a thrombospondin type 1 domain, and a cysteine knot region. CTGF plays important roles in the proliferation and differentiation of chondrocytes, induces angiogenesis, and promotes cell adhesion in fibroblasts, endothelial, and epithelial cells.

Specifications

• Accession Number: P29279
• For Use With (Application): Control, ELISA, Functional Assay, Immunohistochemistry, Western Blot
• Formulation: 10 mM sodium acetate with no preservative; pH 6
• Gene ID (Entrez): 1490
• Molecular Weight (g/mol): 11.2 kDa
• Name: Human CTGF (CCN2)
• Purification Method: Sequential Chromatography
• Quantity: 20 μg
• Endotoxin Concentration: <0.1 ng/ μg
• Gene Alias: 2-Nov; betaIG-M2; CCN family member 2; Ccn2; Cellular communication network factor 2; connective tissue growth factor; connective tissue growth factor precursor; Connective tissue growth factor precursor (CTGF) (FISP-12 protein) (Hypertrophic chondrocyte-specific protein 24); connective tissue growth-related protein; CTGF; CTGF protein; CTGRP; fibroblast inducible secreated protein; fibroblast inducible secreted protein; Fisp12; fisp-12; Hcs24; H-CTGF; hypertrophic chondrocyte-specific gene product 24; hypertrophic chondrocyte-specific protein 24; IBP-8; IGF-binding protein 8; IGFBP8; IGFBP-8; Insulin-like growth factor-binding protein 8; MGC102839; NOV2; Protein FISP-12; similar to connective tissue growth factor precursor
• Common Name: CTGF
• Biological Activity: ED50 = 1 - 2 μg/mL; determined by the dose-dependent proliferation of HUVECs.
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Sequence: GKKCIRTPKI SKPIKFELSG CTSMKTYRAK FCGVCTDGRC CTPHRTTTLP VEFKCPDGEV MKKNMMFIKT CACHYNCPGD NDIFESLYYR KMYGDMA
• Content And Storage: -20°C
• Endotoxin Level: <0.1 ng/μg
• Shipping Condition: Wet Ice
• Expression System: E. coli
• Protein Family: Growth Factors & Receptors
• Protein Form: Recombinant, Ligand
• Form: Lyophilized
• Protein Subtype: Other Proteins
• Research Category: Stem Cell Research, Differentiation
• Product Line: Gibco™
• Purity or Quality Grade: ≥95% by SDS-PAGE and HPLC

Safety and Handling

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