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Fc Receptor Binding Inhibitor anti-Human, Polyclonal, eBioscience™ Non-distribution product as customer accommodation.

Polyclonal Antibody

$88.75 - $153.00

Specifications

Host Species Human
Formulation Aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer, PBS with 0.09% sodium azide; pH 7.2
Purity Purified
Storage Requirements 2° to 8°C, 4° C
For Use With (Application) Flow Cytometric Analysis
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Alert:

Contains Azide


Products 2
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
50-138-03 Non-distribution product as customer accommodation.
View Documents
Invitrogen
14916171
25 tests
Each for $88.75
Add to cart
 
50-112-9053 Non-distribution product as customer accommodation.
View Documents
Invitrogen
14916173
100 tests
Each for $153.00
Add to cart
 
Description

Description

Description: The human FcgammaR-binding inhibitor can be used to inhibit the non-specific Fc-gamma receptor (FcgammaR)-mediated binding of mouse monoclonal antibodies used for flow cytometric analysis of human tissue. Four different classes of Fcgamma receptors have been identified: FcgammaRI (CD64), FcgammaRII (CD32), and FcgammaRIII (CD16), and they are expressed at varying levels in multiple cell lineages including high expression in myeloid, granulocyte and B cells. The biological function of the FcgammaR, including initiation of endocytosis, phagocytosis and antigen presentation, is elicited upon binding of host-immunoglobulin. The extent to which mouse monoclonal antibodies will bind to human FcgammaR varies depending on the isotype of the monoclonal antibody. Furthermore, different monoclonal antibodies of the same isotype will display different binding to human FcgammaRs. Applications Reported: Purified Fc Receptor Binding Inhibitor has been reported for use in flow cytometric analysis. To inhibit the non-specific binding of mouse monoclonal antibodies add the human Fc Receptor Binding Inhibitor to samples and incubate on ice for 20 minutes. Without washing proceed to stain with primary antibody. Applications Tested: Purified human Fc gammaR-binding inhibitor has been pre-titrated and tested by inhibiting binding of fluorochrome-conjugated isotype controls to U937 cells. This can be used at 20 μL per test.

A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 μm post-manufacturing filtered. Four different classes of Fcy receptors have been identified: FcyRI (CD64), FcyRII (CD32), and FcyRIII (CD16), and they are expressed at varying levels in multiple cell lineages including high expression in myeloid, granulocyte and B cells. The biological function of the FcyR, including initiation of endocytosis, phagocytosis and antigen presentation, is elicited upon binding of host-immunoglobulin. The extent to which mouse monoclonal antibodies will bind to human FcyR varies depending on the isotype of the monoclonal antibody. Furthermore, different monoclonal antibodies of the same isotype will display different binding to human FcyRs.
Specifications

Specifications

Human
Purified
Flow Cytometric Analysis
Aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer, PBS with 0.09% sodium azide; pH 7.2
2° to 8°C, 4° C
SDS

For Research Use Only

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