Gibco™ Human Fetuin A/AHSG Recombinant Protein, PeproTech®

Description

Recombinant Protein

Recombinant Human Fetuin A is a heterodimeric protein containing a 282 amino acid A chain and a 27 amino acid B chain. The calculated molecular weight of Recombinant Human Fetuin A is 32.9 kDa. Due to glycosylation, recombinant Fetuin A migrates at an apparent molecular weight of 45-55 kDa by SDS-PAGE run under non-reducing conditions. This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

Alpha2-HS glycoprotein (AHSG), a glycoprotein present in the serum, is synthesized by hepatocytes. The AHSG molecule consists of two polypeptide chains, which are both cleaved from a proprotein encoded from a single mRNA. It is involved in several functions, such as endocytosis, brain development and the formation of bone tissue. The protein is commonly present in the cortical plate of the immature cerebral cortex and bone marrow hemopoietic matrix, and it has therefore been postulated that it participates in the development of the tissues. However, its exact significance is still obscure.

Specifications

• Accession Number: P02765
• For Use With (Application): Functional Assay
• Formulation: protein with no preservative
• Gene ID (Entrez): 197
• Molecular Weight (g/mol): 32.9 kDa
• Name: Human Fetuin A/AHSG
• Quantity: 100 μg
• Source: HEK293 cells
• Regulatory Status: RUO
• Endotoxin Concentration: <1 EU/ μg
• Gene Alias: 59 kDa bone sialic acid-containing protein; A2HS; Aa2-066; AHS; AHSG; alpha 2 HS-glycoprotein alpha 2 (fetuin); alpha 2-HS glycoprotein; alpha-2-HS-glycoprotein; Alpha-2-HS-glycoprotein chain A; Alpha-2-HS-glycoprotein chain B; Alpha-2-Z-globulin; asialofetuin; Ba-alpha-2-glycoprotein; BSP; Countertrypin; FETUA; fetuin; fetuin A; FetuinA; fetuin-A; Glycoprotein PP63; HSGA; phosphorylated N-glycoprotein pp63; pp63; PRO2743
• Common Name: Fetuin A
• Gene Symbol: AHSG
• Biological Activity: Determined by its ability to inhibit Cathespin V cleavage of a fluorogenic peptide substrate Z-LR-AMC (RLUs). The expected IC50 is ≤ 100 nM.
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Sequence: A-Chain: APHGPGLIYR QPNCDDPETE EAALVAIDYI NQNLPWGYKH TLNQIDEVKV WPQQPSGELF EIEIDTLETT CHVLDPTPVA RCSVRQLKEH AVEGDCDFQL LKLDGKFSVV YAKCDSSPDS AEDVRKVCQD CPLLAPLNDT RVVHAAKAAL AAFNAQNNGS NFQLEEISRA QLVPLPPSTY VEFTVSGTDC VAKEATEAAK CNLLAEKQYG FCKATLSEKL GGAEVAVTCT VFQTQPVTSQ PQPEGANEAV PTPVVDPDAP PSPPLGAPGL PPAGSPPDSH VL B-Chain: TVVQPSVG AAAGPVVPPC PGRIRHFKV
• Content And Storage: -20°C
• Expression System: HEK293 cells
• Form: Lyophilized
• Purity or Quality Grade: ≥ 98% by SDS-PAGE gel and HPLC analyses.