Invitrogen™ Human FGF-basic (FGF-2/bFGF) (154 aa) Recombinant Protein

Description

Recombinant Protein

Recombinant human FGF-b 154 (FGF-2) is a non-glycosylated protein containing 154 amino acids. Reconstitute using sterile water at 0.1 mg/mL and centrifuge prior to opening vial. Gently pipet solution down the sides of the vial. DO NOT VORTEX sample. Store reconstituted material at -20°C and add 0.1% BSA for additional stability.

FGF2 (FGFb, fibroblast growth factor basic) belongs to the fibroblast growth factor (FGF) family, and interacts with high-affinity transmembrane receptors to influence cell proliferation and tissue neovascularization. FGF2 exists as five isoforms with distinct intracellular localizations and functions. The 18 kDa isoform is predominantly cytosolic and acts through cell surface receptors, whereas the 22, 22.5, 24 and 34 kDa isoforms are nuclear and may signal independent of transmembrane receptor pathways. In humans, the gene is located on the q arm of chromosome 4. FGF2 has been implicated in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. The mRNA for FGF2 contains multiple polyadenylation sites, and is alternatively translated from non-AUG and AUG initiation codons, resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF. Diseases associated with FGF2 dysfunction include Kaposi Sarcoma and corneal neovascularization.

Specifications

• Accession Number: P09038
• Concentration: 0.1 mg/mL
• For Use With (Application): Control
• Formulation: 10 mM sodium phosphate with 75 mM NaCl and no preservative; pH 7.5
• Gene ID (Entrez): 2247
• Molecular Weight (g/mol): 17.3 kDa
• Name: Human FGF-basic (FGF-2/bFGF) (154 aa)
• pH Range: 7.5
• Quantity: 50 μg
• Source: E. coli
• Regulatory Status: RUO
• Endotoxin Concentration: ≤0.1 EU/μg
• Gene Alias: Basic fibroblast growth factor; basic fibroblast growth factor bFGF; bFGF; FGF; fgf basic; Fgf2; Fgf-2; Fgfb; FGF-b; Fibroblast growth factor; fibroblast growth factor 2; fibroblast growth factor 2 (basic); HBGF-2; Heparin-binding growth factor 2; H-FGF-b-147; H-FGF-b-154; M-FGF-b; Prostatic growth factor; prostatropin
• Common Name: FGF2
• Gene Symbol: FGF2
• Biological Activity: The ED50 determined by NR6R-3T3 Proliferation Assay
• Conjugate: Unconjugated
• Species: E. coli
• Recombinant: Recombinant
• Sequence: MAAGSITTLP ALPEDGGSGA FPPGHFKDPK RLYCKNGGFF LRIHPDGRVD GVREKSDPHI KLQLQAEERG VVSIKGVCAN RYLAMKEDGR LLASKCVTDE CFFFERLESN NYNTYRSRKY TSWYVALKRT GQYKLGSKTG PGQKAILFLP MSAKS
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Expression System: E. coli
• Form: Lyophilized
• Protein Subtype: Recombinant
• Purity or Quality Grade: ≥95%

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov