Invitrogen™ Human Furin Recombinant Protein

Description

Recombinant Protein

RP-062 has an activity of 1 U/ul, where 1 U (a unit of activity) is defined as the amount of furin that can release 1 pmol of AMC from the fluorogenic peptide pERTKR-MCA in 1 min. As a fully functional enzyme, RP-062 can also be used in a variety of enzymatic assays. 1 U (a unit of activity) is defined as the amount of furin that can release 1 pmol of AMC from the fluorogenic peptide pERTKR-MCA in one minute (1 pmol of AMC/min). This furin construct represents amino acid residues 1-713 from human furin convertase protein.

Furin is a membrane-associated, calcium-dependent, serine protease that belongs to the subtilisin-like prohormone convertase (PC) family. Members of this family of cellular enzymes cleave most prohormones and neuropeptide precursors. Numerous other cellular proteins, some viral proteins, and bacterial toxins that are transported by the constitutive secretory pathway are also targeted for maturation by PCs. Furin and other PC family members share structural similarities which include a heterogeneous ∽10 kDa amino-terminal proregion, a highly conserved ∽55 kDa subtilisin-like catalytic domain, and carboxyl-terminal domain that is heterogeneous in length and sequence. These enzymes become catalytically active following proregion cleavage within the appropriate cellular compartment. Furin is the only known PC to possess a transmembrane domain. Cleavage of target proteins occurs at the carboxyl-terminus of the furin consensus sequence, RX(K/R)R. It has been shown that the acidic peptide sequence, C771PSDSEEDEG780, localizes furin to the trans-Golgi-network. Phosphorylation of serine residues within this region modulates intracellular routing of furin protein.

Specifications

• For Use With (Application): Control, Western Blot
• Formulation: 0.02 M Bis-tris with 1 mg/mL BSA and no preservative
• Name: Human Furin
• Quantity: 50 μL
• Source: Non-Human Primate
• Regulatory Status: RUO
• Common Name: Furin Convertase
• Biological Activity: Specific Activity = 1 U/ μL; where 1 U is defined as the amount of furin that can release 1 pmol of AMC from the fluorogenic peptide pERTKR-MCA in 1 min.
• Conjugate: Unconjugated
• Species: Non-human Primate
• Recombinant: Recombinant
• Sequence: Human Furin convertase protein, amino acids 1-713
• Content And Storage: -80°C, Avoid Freeze/Thaw Cycles
• Expression System: Non-Human Primate cells
• Form: Liquid
• Protein Subtype: Recombinant

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov