R&D Systems™ Recombinant Human His6-USP46/UAF1 Complex Protein, CF

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility.

Recombinant Human USP46/UAF1 complex is a Ubiquitin-specific deconjugating enzyme heterodimer. Reaction conditions will need to be optimized for each specific application. We recommend an initial USP46/UAF1 Complex concentration of 20-200 nM when using Ubiquitin-AMC or Ubiquitin-Rhodamine (U-550, U-555) as a substrate. In vitro, USP46/UAF1 complex does not efficiently hydrolyze poly-Ubiquitin chains in the absence of WDR20.

Specifications

• Accession Number: P62068 (USP46), Q8TAF3 (UAF1)
• For Use With (Application): Bioactivity
• Formulation: X mg/mL (X μM) in 50 mM HEPES pH 7.8, 100 mM NaCl, 10% (v/v) Glycerol, 1 mM TCEP
• Molecular Weight (g/mol): M.W. (theoretical): 43 kDa (USP46), 77 kDa (UAF1)
• Quantity: 70 μg
• Source: Spodoptera frugiperda, Sf 21 (baculovirus)-derived human USP46/UAF1 Complex protein Contains a C-terminal 6-His tag
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -70°C as supplied. 3 months, -70°C under sterile conditions after opening.
• Common Name: USP46/UAF1 Complex
• Biological Activity: Recombinant Human USP46/UAF1 complex is a Ubiquitin-specific deconjugating enzyme heterodimer. Reaction conditions will need to be optimized for each specific application. We recommend an initial USP46/UAF1 Complex concentration of 20-200 nM when using Ubiquitin-AMC or Ubiquitin-Rhodamine (U-550, U-555) as a substrate. In vitro, USP46/UAF1 complex does not efficiently hydrolyze poly-Ubiquitin chains in the absence of WDR20.
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Expression System: Spodoptera frugiperda, Sf 21 (baculovirus)-derived Contains a C-terminal 6-His tag
• Purity or Quality Grade: >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie™ Blue stain.
• Protein: USP46/UAF1 Complex