Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More
Learn More
R&D Systems™ Recombinant Human His6-USP46/UAF1 Complex Protein, CF
Click to view available options
Quantity:
70 μg
Description
Recombinant Human USP46/UAF1 complex is a Ubiquitin-specific deconjugating enzyme heterodimer. Reaction conditions will need to be optimized for each specific application. We recommend an initial USP46/UAF1 Complex concentration of 20-200 nM when using Ubiquitin-AMC or Ubiquitin-Rhodamine (U-550, U-555) as a substrate. In vitro, USP46/UAF1 complex does not efficiently hydrolyze poly-Ubiquitin chains in the absence of WDR20.
Specifications
Specifications
Accession Number | P62068 (USP46), Q8TAF3 (UAF1) |
For Use With (Application) | Bioactivity |
Formulation | X mg/mL (X μM) in 50 mM HEPES pH 7.8, 100 mM NaCl, 10% (v/v) Glycerol, 1 mM TCEP |
Molecular Weight (g/mol) | M.W. (theoretical): 43 kDa (USP46), 77 kDa (UAF1) |
Quantity | 70 μg |
Source | Spodoptera frugiperda, Sf 21 (baculovirus)-derived human USP46/UAF1 Complex protein Contains a C-terminal 6-His tag |
Storage Requirements | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -70°C as supplied. 3 months, -70°C under sterile conditions after opening. |
Common Name | USP46/UAF1 Complex |
Biological Activity | Recombinant Human USP46/UAF1 complex is a Ubiquitin-specific deconjugating enzyme heterodimer. Reaction conditions will need to be optimized for each specific application. We recommend an initial USP46/UAF1 Complex concentration of 20-200 nM when using Ubiquitin-AMC or Ubiquitin-Rhodamine (U-550, U-555) as a substrate. In vitro, USP46/UAF1 complex does not efficiently hydrolyze poly-Ubiquitin chains in the absence of WDR20. |
Conjugate | Unconjugated |
Show More |
Product Content Correction
Your input is important to us. Please complete this form to provide feedback related to the content on this product.
Product Title
Spot an opportunity for improvement?Share a Content Correction