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R&D Systems™ Recombinant Human His6-USP46/UAF1 Complex Protein, CF

Catalog No. E586070
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Quantity:
70 μg
This item is not returnable. View return policy
This item is not returnable. View return policy

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility.

Recombinant Human USP46/UAF1 complex is a Ubiquitin-specific deconjugating enzyme heterodimer. Reaction conditions will need to be optimized for each specific application. We recommend an initial USP46/UAF1 Complex concentration of 20-200 nM when using Ubiquitin-AMC or Ubiquitin-Rhodamine (U-550, U-555) as a substrate. In vitro, USP46/UAF1 complex does not efficiently hydrolyze poly-Ubiquitin chains in the absence of WDR20.

Specifications

Accession Number P62068 (USP46), Q8TAF3 (UAF1)
For Use With (Application) Bioactivity
Formulation X mg/mL (X μM) in 50 mM HEPES pH 7.8, 100 mM NaCl, 10% (v/v) Glycerol, 1 mM TCEP
Molecular Weight (g/mol) M.W. (theoretical): 43 kDa (USP46), 77 kDa (UAF1)
Quantity 70 μg
Source Spodoptera frugiperda, Sf 21 (baculovirus)-derived human USP46/UAF1 Complex protein Contains a C-terminal 6-His tag
Storage Requirements Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -70°C as supplied. 3 months, -70°C under sterile conditions after opening.
Common Name USP46/UAF1 Complex
Biological Activity Recombinant Human USP46/UAF1 complex is a Ubiquitin-specific deconjugating enzyme heterodimer. Reaction conditions will need to be optimized for each specific application. We recommend an initial USP46/UAF1 Complex concentration of 20-200 nM when using Ubiquitin-AMC or Ubiquitin-Rhodamine (U-550, U-555) as a substrate. In vitro, USP46/UAF1 complex does not efficiently hydrolyze poly-Ubiquitin chains in the absence of WDR20.
Conjugate Unconjugated
Species Human
Recombinant Recombinant
Expression System Spodoptera frugiperda, Sf 21 (baculovirus)-derived Contains a C-terminal 6-His tag
Purity or Quality Grade >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie™ Blue stain.
Protein USP46/UAF1 Complex
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