Human HMGB1/HMG-1 Antibody, R&D Systems™

Mouse Monoclonal Antibody

Supplier:  R&D Systems MAB1690100

Catalog No. MAB1690100

Description

HMGB1/HMG-1 Monoclonal specifically detects HMGB1/HMG-1 in Human samples. It is validated for Western Blot, Immunohistochemistry, Intracellular Staining by Flow Cytometry, Chromatin Immunoprecipitation (ChIP), Immunocytochemistry, CyTOF-ready.

Specifications

• Antigen: HMGB1/HMG-1
• Classification: Monoclonal
• Conjugate: Unconjugated
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS. with No Preservative
• Gene Alias: Amphoterin, high mobility group box 1, High mobility group protein 1, high mobility group protein B1, high-mobility group (nonhistone chromosomal) protein 1, high-mobility group box 1, HMG1, HMG-1, HMG1DKFZp686A04236, HMG3, SBP-1, Sulfoglucuronyl carbohydrate binding protein
• Host Species: Mouse
• Purification Method: Protein A or G purified from hybridoma culture supernatant
• Regulatory Status: RUO
• Gene ID (Entrez): 3146
• Reconstitution: Reconstitute at 0.5 mg/mL in sterile PBS.
• Content And Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Isotype: IgG2b

• Applications: ChIP Assay, CyTOF, Flow Cytometry, Immunocytochemistry, Immunohistochemistry
• Clone: 115603
• Dilution: Western Blot 0.05 μg/mL, Immunohistochemistry 5-25 μg/mL, Intracellular Staining by Flow Cytometry 2.5 μg/10^6 cells, Chromatin Immunoprecipitation (ChIP) 5 μg/5 x 10^6 cells, Immunocytochemistry 8-25 μg/mL, CyTOF-ready
• Gene Accession No.: P09429
• Gene Symbols: HMGB1
• Immunogen: E. coli-derived recombinant human HMGB1/HMG-1 Gly2-Glu215 (predicted) Accession # P09429
• Quantity: 100 μg
• Primary or Secondary: Primary
• Test Specificity: Detects human HMGB1/HMG-1 in direct ELISAs and Western blots.
• Target Species: Human
• Form: Supernatant