Gibco™ Human ICAM-2 Fc Recombinant Protein, PeproTech®

Description

Recombinant Protein

PeproTech's CHO-derived Recombinant Human ICAM-2 Fc is a glycosylated, disulfide-linked homodimer of 435 amino-acid-residues whose monomer consists of the 202-amino-acid extracellular portion of ICAM-2 fused to the 231-amino-acid length Fc portion of human IgG by two glycine residues. The calculated molecular weight of monomeric CHO cell-derived Recombinant Human ICAM-2 Fc is 48.5 kDa; however, due to glycosylation, it migrates at an apparent molecular weight of approximately 70-80 kDa by SDS-PAGE analysis under reducing conditions. This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

ICAM-2 is a member of the intercellular adhesion molecule (ICAM) family. All ICAM proteins are type I transmembrane glycoproteins, contain 2-9 immunoglobulin-like C2-type domains, and bind to the leukocyte adhesion LFA-1 protein. ICAM-2 may play a role in lymphocyte recirculation by blocking LFA-1-dependent cell adhesion. It mediates adhesive interactions important for antigen-specific immune response, NK-cell mediated clearance, lymphocyte recirculation, and other cellular interactions important for immune response and surveillance. Several transcript variants encoding the same protein have been found for this gene. Diseases associated with ICAM2 include Colon Carcinoma In Situ.

Specifications

• Accession Number: P13598
• For Use With (Application): Functional Assay
• Formulation: protein with no preservative
• Gene ID (Entrez): 3384
• Molecular Weight (g/mol): 48.5 kDa
• Name: Human ICAM-2 Fc
• Quantity: 100 μg
• Source: CHO cells
• Regulatory Status: RUO
• Endotoxin Concentration: <1 EU/ μg
• Gene Alias: CD102; Icam2; ICAM-2; Intercellular adhesion molecule 2; Ly-60; Lymphocyte function-associated AG-1 counter-receptor; RP23-418O11.10-001
• Common Name: ICAM-2
• Gene Symbol: ICAM2
• Biological Activity: Determined by its ability to support the adhesion of PMA-stimulated HSB2 cells to plates pre-coated with ICAM-3 Fc. The ED50 for this effect is 1.0-5.0 ug/ml
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Protein Tag: Fc-tag
• Sequence: SDEKVFEVHV RPKKLAVEPK GSLEVNCSTT CNQPEVGGLE TSLDKILLDE QAQWKHYLVS NISHDTVLQC HFTCSGKQES MNSNVSVYQP PRQVILTLQP TLVAVGKSFT IECRVPTVEP LDSLTLFLFR GNETLHYETF GKAAPAPQEA TATFNSTADR EDGHRNFSCL AVLDLMSRGG NIFHKHSAPK MLEIYEPVSD SQGGPKSCDK THTCPPCPAP ELLGGPSVFL FPPKPKDTLM ISRTPEVTCV VVDVSHEDPE VKFNWYVDGV EVHNAKTKPR EEQYNSTYRV VSVLTVLHQD WLNGKEYKCK VSNKALPAPI EKTISKAKGQ PREPQVYTLP PSRDELTKNQ VSLTCLVKGF YPSDIAVEWE SNGQPENNYK TTPPVLDSDG SFFLYSKLTV DKSRWQQGNV FSCSVMHEAL HNHYTQKSLS LSPGK
• Content And Storage: -20°C
• Expression System: CHO cells
• Form: Lyophilized
• Purity or Quality Grade: ≥ 98% by SDS-PAGE gel and HPLC analyses.