Human IFN-alpha Antibody, R&D Systems™

Mouse Monoclonal Antibody has been used in 2 publications

Supplier:  R&D Systems 211121

Catalog No. 211121

Description

IFN-alpha Monoclonal specifically detects IFN-alpha in Human samples. It is validated for ELISA, Neutralization.

Specifications

• Antigen: IFN-alpha
• Classification: Monoclonal
• Conjugate: Unconjugated
• Formulation: Phosphate-buffered saline (PBS) containing 0.1% bovine serum albumin (BSA) with No Preservative
• Gene Symbols: IFNA2
• Immunogen: Human Leukocyte Interferon
• Quantity: 50 μg
• Primary or Secondary: Primary
• Test Specificity: One neutralization unit is the amount of antiserum required to neutralize one unit of human interferon alpha (Hu-IFN-alpha ) to a 50% endpoint. Interferon was titrated with the use of the cytopathic effect inhibition assay as described [Rubinstein, S., Familletti, P.C., and Pestka, S. (1981) “Convenient Assay for Interferons,” J. Virol . 37, 755-758; Familletti, P.C., Rubinstein, S., and Pestka, S. (1981) “A Convenient and Rapid Cytopathic Effect Inhibition Assay for Interferon,” in Methods in Enzymology, Vol. 78 (S. Pestka, ed.), Academic Press, New York, 387-394]. In this antiviral assay for interferon, about 1 unit/ml of interferon is the quantity necessary to produce a cytopathic effect of 50%. The units are determined with respect to the international reference standard for Hu-IFN-alpha provided by the National Institutes of Health [see Pestka, S. (1986) “Interferon Standards and General Abbreviations,” in Methods in Enzymology (S. Pestka, ed.), Academic Press, New York 119, 14-23].
• Content And Storage: After receipt, the product may be stored at -20degreesC for short-term use ( < 6 months). For long-term storage, we recommend storing the product at -70degreesC or below for retention of full activity. When thawing, the contents of the tube should be apportioned in separate tubes so that freezing and thawing is kept to a minimum. Refreezing should be done on dry ice or in a dry ice/alcohol bath. Further dilution of the product should be in buffers containing protein such as 0.1% bovine serum albumin (BSA).
• Isotype: IgG1

• Applications: ELISA, Neutralization
• Clone: MMHA-11
• Dilution: ELISA, Neutralization
• Gene Alias: IFNA, IFNA2, IFNA2b, IFNalpha, IFN-alphaA, INFA2, interferon alpha 2, LeIF D
• Host Species: Mouse
• Purification Method: Protein A or G purified from hybridoma culture supernatant
• Regulatory Status: RUO
• Gene ID (Entrez): 3440
• Target Species: Human
• Form: Purified