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Invitrogen™ IL-20 Human ELISA Kit
Sandwich ELISA Kit
Supplier: Invitrogen™ EHIL20
Description
The Human Interleukin 20 (Hu IL-20) ELISA quantitates Hu IL-20 in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu IL-20. Principle of the method The Human IL-20 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Cytokines that signal through Class II receptors form a distinct family that includes the interferons and interleukin 10 (IL-10). Recent identification of several IL-10 homologs has defined a cytokine subfamily that includes AK155, IL-19, IL-20, IL-22, and IL-24. IL-20 belongs to the IL-10 family and plays a role in skin inflammation and the development of hematopoietic cells. Little is known about its other biological functions and clinical implications. These cytokines display pro-inflammatory activities in different tissues, including skin.Specifications
Q9NYY1 | |
1 pg/mL | |
Solid-phase sandwich ELISA | |
ELISA Kit | |
Plasma, Serum, Supernatant | |
96 assays | |
ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis | |
50604 | |
<12% | |
Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers | |
96 Tests | |
Inflammation | |
2°C to 8°C | |
Human | |
4 hr. 45 min. |
2.05-500 pg/mL | |
1pg/mL to 500pg/mL | |
Biotin | |
Other Proteins | |
This ELISA antibody pair detects human IL-20. Other species not determined yet. | |
Human | |
Colorimetric Microplate Reader | |
IL-20,IL10D,ZCYTO10 | |
<10% | |
HRP | |
RUO | |
Plasma, 50 μL; Serum, 50 μL; Supernatant, 100 μL | |
UNQ852/PRO1801, IL-20, IL10D, ZCYTO10 | |
1 hr. 20 min. |
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