Gibco™ Human IL-23 Recombinant Protein

Description

Recombinant Protein

Carrier-Free Reconstitution: Lyophilized human IL-23 should be reconstituted in water to a protein concentration of 0.1-1.0 mg/mL. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein, such as 0.1% BSA and store in working aliquots at -80°C to -20°C. Storage: Lyophilized human IL-23 should be stored at 2°C to 8°C, preferably desiccated. Store reconstituted human IL-23 at ≤-20°C (not in a frost-free freezer). Keep freeze-thaw cycles to a minimum.

IL-23 is a heterodimeric cytokine composed of the p40 subunit of IL-12 disulfide-linked with a protein p19. p19, like p35 of IL-12, is biologically inactive by itself. IL-23 interacts with IL-12Rbeta1 and an additional, novel beta2-like receptor subunit with STAT4 binding domain, termed IL-23R. IL-23 is secreted by activated mouse and human dendritic cells. Biological activities of mouse IL-23 are distinct from those of mouse IL-12. Mouse IL-23 was found not to induce significant amounts of IFN-γ. Mouse IL-23 does induce strong proliferation of memory T cells (but not naive T cells), whereas IL-12 has no effect on memory cells. Additionally, mouse IL-23 (but not IL-12) can activate mouse memory T cells to produce the proinflammatory cytokine IL-17. Human IL-23 has biological properties which are less distinct from human IL-12; human IL-23 induces proliferation of memory T cells and induces moderate levels of IFN-γ production by naive and memory T cells, as compared to IL-12.

Specifications

• Accession Number: Q9NPF7
• For Use With (Application): Control, ELISA, Functional Assay, Immunohistochemistry, Western Blot
• Formulation: protein with no preservative
• Gene ID (Entrez): 51561
• Molecular Weight (g/mol): 55 kDa
• Name: Human IL-23
• Purification Method: Sequential Chromatography
• Quantity: 10 μg
• Endotoxin Concentration: <0.1 ng/ μg
• Gene Alias: il 23; IL 23 A; IL12B; Il-12 b; Il12p40; Il-12p40; il23; IL-23; IL-23 subunit alpha; IL23A; IL-23 A; IL-23-A; IL23P19; IL-23p19; ILN; Interleukin; interleukin 12 B; interleukin 23 p19 subunit; interleukin 23 subunit alpha; interleukin 23, alpha subunit p19; interleukin-23 subunit alpha; Interleukin-23 subunit p19; interleukin-six, G-CSF related factor; JKA3 induced upon T-cell activation; MGC79388; P19; p40; RP23-388G23.1; SGRF; UNQ2498/PRO5798
• Common Name: IL-23 p19
• Biological Activity: ED50 ≤ 20 ng/mL; determined by the dose-dependent induction of IL-17 secretion by mouse splenocytes.
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Sequence: Human IL-23 recombinant protein is a heterodimer containing a p40 and p19 subunit
• Content And Storage: 4°C
• Classification: Carrier-Free
• Activity: 0.3 ng/ml
• Endotoxin Level: <1 EU/ml
• Shipping Condition: Wet Ice
• Expression System: HEK293 cells
• Protein Family: Cytokines & Receptors
• Protein Form: Recombinant, Ligand
• Form: Lyophilized
• Protein Subtype: Interleukins
• Research Category: Inflammation, Signal Transduction
• Product Line: Gibco™
• Purity or Quality Grade: ≥95% by SDS-PAGE and HPLC

Safety and Handling

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