Human IL-13 Recombinant Protein

Description

Recombinant Protein

Human Interleukin 13 (IL-13) Recombinant Protein, RIL135, from Invitrogen.

IL-13 is an immunoregulatory cytokine produced primarily by activated Th2 cells, and is involved in regulating inflammatory and immune responses. IL-13 down-regulates macrophage activity, thereby inhibiting the production of pro-inflammatory cytokines and chemokines, and is involved in allergen-induced asthma through mechanisms independent of IgE and eosinophils. IL-13 also regulates several stages of B cell maturation and differentiation, up-regulates CD23 and MHC class II expression, and promotes IgE isotype switching of B cells. Structurally, IL-13 closely resembles IL-3, IL-4, IL-5 and GM-CSF. The gene encoding IL-13, IL3, IL5, IL4, and CSF2 form a cytokine gene cluster on chromosome 5q, with the IL-13 gene particularly close to IL4. Purity: >98% pure by SDS-PAGE and HPLC analysis. Activity: One unit of activity is <1.0ng/mL, as determined in a proliferation assay of human TF-1 cells, corresponding to a specific activity of >1x10^6 units/mg. Use: IL-13 exerts activity in the range of 0.1 to 10ng/mL for most in vitro applications. Reconstitution and Dilution: Reconstitute in distilled water to a concentration of 100ug/mL. After reconstitution, dilute cytokine in a neutral buffer containing carrier protein such as 5-10% FBS or 1-4% high purity BSA (fatty acid free fraction V). Lyophilized product is stable until expiration if stored desiccated below 0°C. Cytokines diluted as indicated above should be divided into aliquots and stored at -70°C. Recombinant proteins should be stored at concentrations >500ng/mL to reduce loss of activity.

Specifications

• Accession Number: P35225
• For Use With (Application): Control, ELISA, Immunoprecipitation, Western Blot
• Formulation: protein with no preservative
• Gene ID (Entrez): 3596
• Name: Human IL-13
• Quantity: 5 μg
• Storage Requirements: -20°C, Avoid Freeze/Thaw Cycles
• Regulatory Status: RUO
• Gene Alias: interleukin-13
• Common Name: Human IL-13
• Gene Symbol: IL13
• Specific Reactivity: Human
• Conjugate: Unconjugated
• Cross Reactivity: Hu
• Species: E. coli
• Recombinant: Recombinant
• Form: Lyophilized
• Protein Subtype: Recombinant
• Purity or Quality Grade: purified