Invitrogen™ Human ISG15 Recombinant Protein

Description

Recombinant Protein

It is recommended that the protein be aliquoted for optimal storage. Has a calculated molecular mass of 17.2 kDa. It migrates as an approximately 15 kDa band in SDS-PAGE under reducing conditions. Reconstitute with sterile deionized water up 0.53mg/mL. Concentration is measured by UV.

Ubiquitin-like protein ISG15 plays a key role in the innate immune response to viral infection either via its conjugation to a target protein (ISGylation) or via its action as a free or unconjugated protein. It is secreted from monocytes in response to type I interferons and causes natural killer (NK)-cell proliferation and an augmentation of non-MCH (major histocompatibility complex)-restricted cytotoxicity. Synthesis is stimulated by IFN-alpha or IFN-beta or IFN-omega, but not IFN-gamma. ISG15 expression is also induced by overexpression of interferon regulatory factors that participate in transcriptional regulation of IFN genes, and by influenza B virus. ISG15 is secreted by cell lines of monocyte, T-lymphocyte, B-lymphocyte, human fibroblasts, and epithelial origins. Enhancement of NK cell proliferation, augmentation of non-major histocompatibility complex-restricted cytotoxicity, and induction of IFN-gamma from T cells identify ISG15 as a member of the cytokine cascade and suggest that it may be responsible for amplifying and directing some of the immunomodulatory effects of IFN-alpha or IFN-beta. ISG15 has has also been shown to function intracellularly as a ubiquitin homolog. Mutations affecting the gene can result in Immunodeficiency 38, with basal ganglia calcification (IMD38).

Specifications

• Accession Number: P05161
• For Use With (Application): Control
• Format: Lyophilized
• Formulation: 50 mM tris with 0.01% Tween 80, 5% mannitol, 5% trehalose and no preservative; pH 8
• Gene ID (Entrez): 9636
• Molecular Weight (g/mol): 15 kDa
• Name: Human ISG15
• Purification Method: SDS-PAGE
• Quantity: 1 mg
• Regulatory Status: RUO
• Gene Alias: G1P2; hUCRP; IFI15; IGI15; IMD38; interferon, alpha-inducible protein; interferon, alpha-inducible protein (clone IFI-15 K); interferon-induced 15 kDa protein; interferon-induced 15-KDa protein; interferon-induced 17 kDa protein; interferon-induced 17-kDa/15-kDa protein; interferon-stimulated protein (15 kDa); interferon-stimulated protein 15; interferon-stimulated protein, 15 kDa; IP17; Irfp; Isg15; ISG15 ubiquitin like modifier; ISG15 ubiquitin-like modifier; ubiquitin cross-reactive protein; Ubiquitin-like protein ISG15; UCRP
• Common Name: ISG15
• Gene Symbol: ISG15
• Product Type: Protein
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Sequence: Mature form of human ISG15, amino acids Met1-Gly157 (Accession # AAH09507.1)
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Expression System: E. coli
• Form: Lyophilized
• Purity or Quality Grade: >95% as determined by SDS-PAGE.