Invitrogen™ Human JAM-C ELISA Kit

Description

ELISA

Human JAM-C quantitates human JAM-C in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant human JAM-C.

JAM3 is involved in tight junctions and represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. The protein encoded by this immunoglobulin superfamily gene member is localized in the tight junctions between high endothelial cells. Unlike other proteins in this family, the this protein is unable to adhere to leukocyte cell lines and only forms weak homotypic interactions. The encoded protein is a member of the junctional adhesion molecule protein family and acts as a receptor for another member of this family.

Specifications

• Accession Number: 1110002N23Rik; JAM-2; JAM3; JAM-3; JAMC; JAM-C; Jcam3; junction adhesion molecule 3; junction cell adhesion molecule 3; junction cell adhesion molecule C; junctional adhesion molecule 3; Junctional adhesion molecule C; sJAM-C; Soluble form of JAM-C; UNQ859/PRO1868
• Assay Range: 0.12 to 30 ng/mL
• Assay Sensitivity: 0.12 ng/mL
• Conjugate: HRP
• Product Type: ELISA
• Sample Type: Plasma, Serum, Supernatant
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 83700
• Gene Symbol: JAM3
• Interassay CV: <12%
• Intraassay CV: <10
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: HRP
• Quantity: 5 x 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
• Storage Requirements: 2°C to 8°C
• Target: JAM3
• Test Time: 1 hrs 20 mins
• Total Assay Time: 4 hrs 45 mins

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov