Invitrogen™ Human Kallikrein 11 His-tag Recombinant Protein

Description

Recombinant Protein

It is recommended that the protein be aliquoted for optimal storage. It is recommended that sterile water (250 μL) be added to the vial to prepare a stock solution of 0.2 mg/mL. Concentration is measured by UV.

Hippostasin, also known as KLK11, is a kallikrein-like serine protease, which has two alternatively spliced isoforms, brain-type and prostate-type. Hippostasin plays a role in the prostate, including reproductive and/or tumorigenic functions. Elevated serum levels of KLK11 were found in 70% of women with ovarian cancer and in 60% of men with prostate cancer. Analysis of the KLK11 biomarker in serum may aid in the diagnosis and monitoring of ovarian and prostatic carcinoma.

Specifications

• Accession Number: Q9UBX7
• For Use With (Application): Functional Assay
• Format: Lyophilized
• Formulation: PBS with 0.01% mannitol, 0.01% Tween 80, 5-8% trehalose and no preservative; pH 7.4
• Gene ID (Entrez): 11012
• Molecular Weight (g/mol): 40 kDa
• Name: Human Kallikrein 11 His-tag
• Purification Method: SDS-PAGE
• Quantity: 50 μg
• Regulatory Status: RUO
• Endotoxin Concentration: <1.0 EU/μg
• Gene Alias: 2310015I08Rik; Hippostasin; hK11; kallikrein 11; kallikrein related peptidase 11; kallikrein related-peptidase 11; Kallikrein11; kallikrein-11; Kallikrein-11 inactive chain 1; Kallikrein-11 inactive chain 2; kallikrein-related peptidase 11; KLK11; protease, serine, 20; Prss20; Serine protease 20; TLSP; Trypsin-like protease; UNQ649/PRO1279
• Common Name: Kallikrein 11
• Gene Symbol: Klk11
• Biological Activity: Measured by its ability to cleave a colorimetric peptide substrate D-Val-Leu-Lys-ThioBenzyl ester (VLK-SBzl), in the presence of 5,5'Dithio-bis (2-nitrobenzoic acid) (DTNB) (Edwards, K.M. et al.,1999, J. Biol. Chem. 274: 30468). The specific activity is >200 pmoles/min/ μg. (Activation description: The proenzyme needs to be activated by Thermolysin for an activated form)
• Product Type: Protein
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Protein Tag: His-tag
• Sequence: Human KLK11 isoform 1, amino acids Met1-Asn250 (Accession # NP_006844.1) with a C-terminal His-tag
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Activity: Measured by its ability to cleave a colorimetric peptide substrate D-Val-Leu-Lys-ThioBenzyl ester (VLK-SBzl), in the presence of 5,5'Dithio-bis (2-nitrobenzoic acid) (DTNB) (Edwards, K.M. et al.,1999, J. Biol. Chem. 274: 30468). The specific activity is >200 pmoles/min/ μg. (Activation description: The proenzyme needs to be activated by Thermolysin for an activated form)
• Expression System: HEK293 cells
• Form: Lyophilized
• Purity or Quality Grade: >90% as determined by SDS-PAGE.