Invitrogen™ Human LAG-3 (CD223) Recombinant Protein, Biotin

Description

Recombinant Protein

It is recommended that the protein be aliquoted for optimal storage. It is recommended that sterile water (400 μL) be added to the vial to prepare a stock solution of 0.25 mg/mL. Concentration is measured by UV.

LAG-3 is a 70-kDa surface glycoprotein belonging to the Ig superfamily with homology to CD4. LAG-3 binds to MHC class II with higher affinity than CD4 and is thought to be involved in the negative regulation of T cell activation and homeostatic proliferation. Surface expression of LAG-3 has been reported on activated T cells (including regulatory T cells) and NK cells. CD8+ T cells usually express LAG-3 at significantly higher levels than CD4+ T cells. Coexpression of LAG-3 and CD49b has been proposed to identify human and mouse Type 1 regulatory T cells (Tr1 cells).

Specifications

• Accession Number: P18627
• Concentration: 0.25 mg/mL
• For Use With (Application): ELISA
• Format: Lyophilized
• Formulation: PBS with 0.01% Tween 80, 5% mannitol, 5% trehalose and no preservative; pH 7.4
• Gene ID (Entrez): 3902
• Molecular Weight (g/mol): 47.0 kDa
• Name: Human LAG-3 (CD223)
• Purification Method: SDS-PAGE
• Quantity: 20 μg
• Regulatory Status: RUO
• Endotoxin Concentration: <1.0 EU/ μg
• Gene Alias: Activation-induced cytidine deaminase-linked autoimmunity protein; Aida; CD223; FDC; LAG3; LAG-3; Ly66; lymphocyte activating 3; lymphocyte activation gene 3 protein; lymphocyte-activation gene 3; Secreted lymphocyte activation gene 3 protein; sLAG 3; sLAG3; sLAG-3; soluble LAG 3 lymphocyte activating 3; soluble LAG3
• Common Name: LAG-3 (CD223)
• Gene Symbol: LAG3
• Biological Activity: Immobilized Human LAG3, Biotinylated (at 2 μg/mL (100 μL/well), EC50 is 5-15 ng/mL Can bind Human FGL1 (hFc Tag), the EC50 is 20-65 ng/mL)
• Product Type: Protein
• Conjugate: Biotin
• Recombinant: Recombinant
• Sequence: Human LAG3, amino acids Met1-Arg440 (Accession # NP_002277.4)
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Activity: Immobilized Human LAG3, Biotinylated (at 2 μg/mL (100 μL/well) can bind Human FGL1 (hFc Tag), the EC50 is 150-700 ng/mL)
• Expression System: HEK293 cells
• Form: Lyophilized
• Purity or Quality Grade: ≥90% as determined by SDS-PAGE.