Gibco™ Human LIF Recombinant Protein

Description

Recombinant Protein

Reconstitution: Centrifuge the vial briefly, before opening to bring the contents to the bottom. Reconstitute the lyophilized protein in sterile, distilled water or appropriate buffered solution containing 0.1% BSA to regain full activity. Apportion there constituted protein into working aliquots and store at -20°C. Make any further dilutions of the reconstituted protein in buffered solution containing a carrier protein, such as PBS with 0.1% BSA. Storage: Store the lyophilized protein at 2-8°C, preferably desiccated. Upon reconstitution, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles.

Leukemia inhibitory factor (LIF) is a 20 kDa protein that belongs to the IL-6 receptor family. It binds to a heterodimeric membrane receptor made up of a LIF-specific subunit, gp190 or LIFR, and the subunit gp130, which is shared with the other members of the IL-6 family. LIF expression has been observed in various tissues including thymus, lung, and neuronal tissue. LIF can be up-regulated by pro-inflammatory cytokines such as TNFα and IL-17, and elevated levels of LIF have been found in cases of rheumatoid arthritis, neural injury, systemic inflammation, and tuberculosis. LIF displays diverse biological effects, but is best known for its ability to inhibit the differentiation of embryonic stem cells in mice and contribute to stem cell self-renewal.Human and mouse LIF share 79% sequence homology and exhibit cross-species activity. However, LIF inhibition of stem cell differentiation appears to be mouse-specific.

Specifications

• Accession Number: P15018
• For Use With (Application): Control, ELISA, Functional Assay, Immunohistochemistry, Western Blot
• Formulation: protein with no preservative
• Gene ID (Entrez): 3976
• Molecular Weight (g/mol): 21 kDa
• Name: Human LIF
• Purification Method: Sequential Chromatography
• Quantity: 1000 μg
• Endotoxin Concentration: <0.1 ng/μg
• Gene Alias: CDF; cholinergic differentiation factor; cholinergic neuronal differentiation factor; d factor; DIA; differentiation inhibitory activity; differentiation-inducing factor; differentiation-stimulating factor; Emfilermin; hepatocyte-stimulating factor III; HILDA; human interleukin in DA cells; leukemia inhibitory factor; leukemia inhibitory factor (cholinergic differentiation factor); LIF; Melanoma-derived LPL inhibitor; MLPLI; myeloid leukaemia inhibitory factor
• Common Name: LIF
• Gene Symbol: LIF
• Biological Activity: ED50 = 0.05 ng/mL; determined by the dose-dependent proliferation of human TF-1 cells.
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Sequence: PLPITPVNAT CAIRHPCHNN LMNQIRSQLA QLNGSANALF ILYYTAQGEP FPNNLDKLCG PNVTDFPPFH ANGTEKAKLV ELYRIVVYLG TSLGNITRDQ KILNPSALSL HSKLNATADI LRGLLSNVLC RLCSKYHVGH VDVTYGPDTS GKDVFQKKKL GCQLLGKYKQ IIAVLAQAF
• Content And Storage: 4°C
• Activity: 0.5 ng/ml
• Bioassay: Dose-dependent proliferation of TF-1 cells
• Shipping Condition: Dry Ice
• Expression System: E. coli
• Protein Family: Cytokines & Receptors
• Protein Form: Recombinant, Ligand
• Form: Lyophilized
• Protein Subtype: Other Cytokines
• Research Category: Oncology
• Product Line: Gibco™
• Purity or Quality Grade: ≥95% by SDS-PAGE
• Protein: Leukemia inhibitory factor