Invitrogen™ Human MAST4 Control Fragment Recombinant Protein

Description

Recombinant Protein

Highest antigen sequence indentity to the following orthologs: Mouse (60%), Rat (60%). This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-51407 (PA5-51407. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.

The phosphorylation and dephosphorylation of proteins on serine and threonine residues is an essential means of regulating a broad range of cellular functions in eukaryotes, including cell division, homeostasis and apoptosis. A group of proteins that are intimately involved in this process are the serine/threonine(Ser/Thr) protein kinases. MAST4 (microtubule associated serine/threonine kinase family member 4), also known as KIAA0303, is a 2,626 amino acid protein that localizes to the cytoplasm and contains one protein kinase domain, as well as one PDZ domain and one AGC-kinase C-terminal domain. Expressed at high levels in most adult tissues, including testis, colon and small intestine, MAST4 uses magnesium as a cofactor to catalyze the ATP-dependent phosphorylation of target proteins. Multiple isoforms of MAST4 exist due to alternative splicing events.

Specifications

• Accession Number: O15021
• Concentration: ≥5.0 mg/mL
• For Use With (Application): Blocking Assay, Control
• Formulation: 1 M urea, PBS with no preservative; pH 7.4
• Gene ID (Entrez): 375449
• Name: Human MAST4 Control Fragment
• Quantity: 100 μL
• Regulatory Status: RUO
• Gene Alias: 4930420O11Rik; AI642422; KIAA0303; MAST4; microtubule associated serine/threonine kinase family member 4; microtubule-associated serine/threonine-protein kinase 4; mKIAA0303; RGD1310139
• Common Name: MAST4
• Gene Symbol: MAST4
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Protein Tag: His-ABP-tag
• Sequence: PEKQESHQKSHGPGSDLENFALFKLEEREKKVYPKAVERSSTFENKASMQEAPPLGSLLKDALHKQASVRASEGAMSDGPVPAEHRQGGGDFRRAPAPGTLQDGLCHS
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Expression System: E. coli
• Form: Liquid
• Purity or Quality Grade: >80% by SDS-PAGE and Coomassie blue staining