Human/Mouse Cleaved Caspase-3 (Asp175) Antibody, R&D Systems™

Rabbit Monoclonal Antibody has been used in 52 publications

Supplier:  R&D Systems MAB835SP

Catalog No. MAB835SP

Description

Caspase-3 Monoclonal specifically detects Caspase-3 in Human, Mouse samples. It is validated for Western Blot, Immunohistochemistry, Intracellular Staining by Flow Cytometry, Immunocytochemistry.

Specifications

• Antigen: Caspase-3
• Classification: Monoclonal
• Concentration: LYOPH
• Dilution: Western Blot 0.5 ug/mL, Immunohistochemistry 0.3-25 ug/mL, Intracellular Staining by Flow Cytometry 0.25 ug/10^6 cells, Immunocytochemistry 8-25 ug/mL
• Gene Accession No.: U26943
• Gene Symbols: CASP3
• Immunogen: KLH-conjugated human Caspase-3 synthetic peptide CRGTELDCGIETD Accession # U26943
• Quantity: 25 μg
• Primary or Secondary: Primary
• Test Specificity: Detects human and mouse Caspase-3 cleaved at Asp175. No cross-reactivity was detected with the full-length procaspase-3 or other caspases.
• Target Species: Human, Mouse
• Form: Purified

• Applications: Western Blot, Immunohistochemistry, Flow Cytometry, Immunocytochemistry
• Clone: 269518
• Conjugate: Unconjugated
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS. with No Preservative
• Gene Alias: Apopain, apoptosis-related cysteine protease, CASP3, CASP-3, caspase 3, apoptosis-related cysteine peptidase, caspase-3, Caspase3, CPP32, CPP-32, CPP32B, CPP32SREBP cleavage activity 1, Cysteine protease CPP32, EC 3.4.22, EC 3.4.22.56, LICE-1, PARP cleavage protease, procaspase3, Protein Yama, SCA-1, Yama
• Host Species: Rabbit
• Purification Method: Protein A or G purified from hybridoma culture supernatant
• Regulatory Status: RUO
• Gene ID (Entrez): 836
• Reconstitution: Reconstitute at 0.5 mg/mL in sterile PBS.
• Content And Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Isotype: IgG

For Research Use Only