Human/Mouse Frizzled-4 Antibody, R&D Systems™

Rat Monoclonal Antibody has been used in 3 publications

Supplier:  R&D Systems MAB194SP

Catalog No. MAB194SP

Description

Frizzled-4 Monoclonal specifically detects Frizzled-4 in Human, Mouse samples. It is validated for Western Blot, Flow Cytometry, Immunocytochemistry, CyTOF-ready.

Specifications

• Antigen: Frizzled-4
• Classification: Monoclonal
• Concentration: LYOPH
• Dilution: Western Blot 1 ug/mL, Flow Cytometry 2.5 ug/10^6 cells, Immunocytochemistry 8-25 ug/mL, CyTOF-ready
• Gene Accession No.: Q61088
• Gene Symbols: FZD4
• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Frizzled-4 Phe37-Glu180 Accession # Q61088
• Quantity: 25 μg
• Primary or Secondary: Primary
• Test Specificity: Detects human and mouse Frizzled-4 in Western blots. In Western blots, no cross-reactivity with recombinant mouse (rm) Frizzled-1, -2, -3, -6, -7, -8, or recombinant human Frizzled-5 is observed. Detects Frizzled-4 by flow cytometry and immunohistochemistry on mouse differentiated embryoid bodies derived from the mouse embryonic cell line, D3.
• Target Species: Human, Mouse
• Form: Purified

• Applications: Western Blot, Flow Cytometry, Immunocytochemistry, CyTOF
• Clone: 145901
• Conjugate: Unconjugated
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS. with No Preservative
• Gene Alias: CD344, CD344 antigen, EVR1, exudative vitreoretinopathy 1, FEVR, frizzled (Drosophila) homolog 4, frizzled homolog 4 (Drosophila), frizzled-4, Frizzled4, Fz-4, FZD4, FZD4S, FzE4, GPCR, hFz4, MGC34390, WNT receptor frizzled-4
• Host Species: Rat
• Purification Method: Protein A or G purified from hybridoma culture supernatant
• Regulatory Status: RUO
• Gene ID (Entrez): 8322
• Reconstitution: Reconstitute at 0.5 mg/mL in sterile PBS.
• Content And Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Isotype: IgG2a