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Human/Mouse IL-12/IL-35 p35 Antibody, R&D Systems™
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Catalog No. MAB1570SP
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Catalog No. Quantity
MAB1570SP 25 μg
MAB1570100 100 μg
MAB1570500 500 μg
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Mouse Monoclonal Antibody has been used in 12 publications

IL-12/IL-35 p35 Monoclonal specifically detects IL-12/IL-35 p35 in Human, Mouse samples. It is validated for Western Blot, Intracellular Staining by Flow Cytometry, CyTOF-ready.

Specifications

Antigen IL-12/IL-35 p35
Applications Western Blot, Flow Cytometry, CyTOF
Classification Monoclonal
Clone 27537
Concentration LYOPH
Conjugate Unconjugated
Dilution Western Blot 1 ug/mL, Intracellular Staining by Flow Cytometry 2.5 ug/10^6 cells, CyTOF-ready
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS. with No Preservative
Gene Accession No. P29459
Gene Symbols IL12A
Host Species Mouse
Immunogen S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL-12/IL-35 p35 Arg23-Ser219 Accession # P29459
Purification Method Protein A or G purified from ascites
Quantity 25 μg
Regulatory Status RUO
Primary or Secondary Primary
Gene ID (Entrez) 3592
Test Specificity Detects human IL-12/IL-35 p35 in direct ELISAs and Western blots. Detects the p35 subunit either as part of a p40/p35 heterodimer or as a free subunit after reduction of the heterodimer. This antibody does not recognize IL-12 p40 homodimers but shows strong cross-reactivity with the p35 subunits from porcine and mouse systems.
Reconstitution Reconstitute at 0.5 mg/mL in sterile PBS.
Target Species Human, Mouse
Content And Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Form Purified
Isotype IgG1
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