Human/Mouse IL-12/IL-35 p35 Antibody, R&D Systems™

Mouse Monoclonal Antibody has been used in 12 publications

Supplier:  R&D Systems MAB1570SP

Catalog No. MAB1570SP

Description

IL-12/IL-35 p35 Monoclonal specifically detects IL-12/IL-35 p35 in Human, Mouse samples. It is validated for Western Blot, Intracellular Staining by Flow Cytometry, CyTOF-ready.

Specifications

• Antigen: IL-12/IL-35 p35
• Classification: Monoclonal
• Concentration: LYOPH
• Dilution: Western Blot 1 ug/mL, Intracellular Staining by Flow Cytometry 2.5 ug/10^6 cells, CyTOF-ready
• Gene Accession No.: P29459
• Host Species: Mouse
• Purification Method: Protein A or G purified from ascites
• Regulatory Status: RUO
• Gene ID (Entrez): 3592
• Reconstitution: Reconstitute at 0.5 mg/mL in sterile PBS.
• Content And Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Isotype: IgG1

• Applications: Western Blot, Flow Cytometry, CyTOF
• Clone: 27537
• Conjugate: Unconjugated
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS. with No Preservative
• Gene Symbols: IL12A
• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL-12/IL-35 p35 Arg23-Ser219 Accession # P29459
• Quantity: 25 μg
• Primary or Secondary: Primary
• Test Specificity: Detects human IL-12/IL-35 p35 in direct ELISAs and Western blots. Detects the p35 subunit either as part of a p40/p35 heterodimer or as a free subunit after reduction of the heterodimer. This antibody does not recognize IL-12 p40 homodimers but shows strong cross-reactivity with the p35 subunits from porcine and mouse systems.
• Target Species: Human, Mouse
• Form: Purified