Human/Mouse/Rat Phospho-Akt (S473) Pan Specific Antibody, R&D Systems™

Rabbit Polyclonal Antibody has been used in 25 publications

Supplier:  R&D Systems AF887SP

Catalog No. AF887SP

Description

AKT Polyclonal specifically detects AKT in Human, Mouse, Rat samples. It is validated for Western Blot, Simple Western, Immunohistochemistry, Intracellular Staining by Flow Cytometry, CyTOF-ready.

Specifications

• Antigen: AKT (p Ser473)
• Classification: Polyclonal
• Conjugate: Unconjugated
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS. with No Preservative
• Gene Symbols: AKT1
• Immunogen: Phosphopeptide containing human, mouse, and rat Akt1 S473 site
• Quantity: 25 μg
• Primary or Secondary: Primary
• Test Specificity: Detects human, mouse and rat Akt1, Akt2 and Akt3, when phosphorylated at S473, S474 and S472, respectively.
• Target Species: Human, Mouse, Rat
• Isotype: IgG

• Applications: Western Blot, Immunohistochemistry, Flow Cytometry, CyTOF
• Concentration: LYOPH
• Dilution: Western Blot 0.5 ug/mL, Simple Western 5 ug/mL, Immunohistochemistry 5-15 ug/mL, Intracellular Staining by Flow Cytometry 2.5 ug/10^6 cells, CyTOF-ready
• Gene Alias: RAC-ALPHA, AKT, AKT serine/threonine kinase 1, PKB, PKB alpha, PKB beta, PKB gamma, PKB-ALPHA, PRKBA, RAC, RAC-alpha, RAC-beta, RAC-gamma
• Host Species: Rabbit
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Gene ID (Entrez): 207
• Reconstitution: Reconstitute at 0.2 mg/mL in sterile PBS.
• Content And Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

For Research Use Only