Human/Mouse/Rat Phospho-ERK1(T202/Y204)/ERK2 (T185/Y187) Antibody, R&D Systems™

Rabbit Polyclonal Antibody

Supplier:  R&D Systems AF1018SP

Catalog No. AF1018SP

Description

ERK1/ERK2 Polyclonal specifically detects ERK1/ERK2 in Human, Mouse, Rat samples. It is validated for Western Blot, Simple Western, Flow Cytometry, Immunohistochemistry, CyTOF-ready.

Specifications

• Antigen: ERK1/ERK2 (p Thr185, p Tyr187, p Thr202, p Tyr204)
• Classification: Polyclonal
• Conjugate: Unconjugated
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS. with No Preservative
• Immunogen: Phosphopeptide containing ERK1 T202/Y204 sites
• Quantity: 25 μg
• Primary or Secondary: Primary
• Reconstitution: Reconstitute at 0.2 mg/mL in sterile PBS.
• Content And Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

• Applications: Western Blot, Flow Cytometry, Immunohistochemistry, CyTOF
• Concentration: LYOPH
• Dilution: Western Blot 0.1 ug/mL, Simple Western 5 ug/mL, Flow Cytometry 2.5 ug/10^6 cells, Immunohistochemistry 5-15 ug/mL, CyTOF-ready
• Host Species: Rabbit
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Test Specificity: Detects human, mouse and rat Phospho-ERK1/ERK2 when dually phosphorylated at T202/Y204 and T185/Y187, respectively.
• Target Species: Human, Mouse, Rat
• Isotype: IgG

For Research Use Only