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Human/Mouse/Rat Phospho-ERK1(T202/Y204)/ERK2 (T185/Y187) Antibody, R&D Systems™
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Catalog No. AF1018SP
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25 μg
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AF1018SP 25 μg
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Description

Rabbit Polyclonal Antibody

ERK1/ERK2 Polyclonal specifically detects ERK1/ERK2 in Human, Mouse, Rat samples. It is validated for Western Blot, Simple Western, Flow Cytometry, Immunohistochemistry, CyTOF-ready.

Specifications

Antigen ERK1/ERK2 (p Thr185, p Tyr187, p Thr202, p Tyr204)
Applications Western Blot, Flow Cytometry, Immunohistochemistry, CyTOF
Classification Polyclonal
Concentration LYOPH
Conjugate Unconjugated
Dilution Western Blot 0.1 ug/mL, Simple Western 5 ug/mL, Flow Cytometry 2.5 ug/10^6 cells, Immunohistochemistry 5-15 ug/mL, CyTOF-ready
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS. with No Preservative
Host Species Rabbit
Immunogen Phosphopeptide containing ERK1 T202/Y204 sites
Purification Method Affinity Purified
Quantity 25 μg
Regulatory Status RUO
Primary or Secondary Primary
Test Specificity Detects human, mouse and rat Phospho-ERK1/ERK2 when dually phosphorylated at T202/Y204 and T185/Y187, respectively.
Reconstitution Reconstitute at 0.2 mg/mL in sterile PBS.
Target Species Human, Mouse, Rat
Content And Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Isotype IgG
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