Human/Mouse/Rat Phospho-ERK1 (T202/Y204)/ERK2 (T185/Y187) Antibody, R&D Systems™

Rabbit Monoclonal Antibody has been used in 7 publications

Supplier:  R&D Systems MAB1018SP

Catalog No. MAB1018SP

Description

ERK1/ERK2 Monoclonal specifically detects ERK1/ERK2 in Human, Mouse, Rat samples. It is validated for Western Blot, Simple Western, Intracellular Staining by Flow Cytometry, Immunocytochemistry, CyTOF-ready.

Specifications

• Antigen: ERK1/ERK2 (p Tyr187, p Tyr204, p Thr185, p Thr202)
• Classification: Monoclonal
• Concentration: LYOPH
• Dilution: Western Blot 0.5 ug/mL, Simple Western 5 ug/mL, Intracellular Staining by Flow Cytometry 2.5 ug/10^6 cells, Immunocytochemistry 8-25 ug/mL, CyTOF-ready
• Host Species: Rabbit
• Purification Method: Protein A or G purified from hybridoma culture supernatant
• Regulatory Status: RUO
• Test Specificity: Detects human, mouse, and rat ERK1 and ERK2 dually phosphorylated at T202/Y204 or T185/Y187, respectively.
• Target Species: Human, Mouse, Rat
• Form: Purified

• Applications: Western Blot, Flow Cytometry, Immunocytochemistry, CyTOF
• Clone: 269434
• Conjugate: Unconjugated
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS. with No Preservative
• Immunogen: Phosphopeptide containing ERK1 T202/Y204 site
• Quantity: 25 μg
• Primary or Secondary: Primary
• Reconstitution: Reconstitute at 0.5 mg/mL in sterile PBS.
• Content And Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Isotype: IgG

For Research Use Only