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Human/Mouse/Rat Phospho-ERK1 (T202/Y204)/ERK2 (T185/Y187) Antibody, R&D Systems™
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Catalog No. MAB1018SP
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25 μg
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MAB1018SP 25 μg
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Catalog No. MAB1018SP Supplier R&D Systems Supplier No. MAB1018SP
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Description

Rabbit Monoclonal Antibody has been used in 7 publications

ERK1/ERK2 Monoclonal specifically detects ERK1/ERK2 in Human, Mouse, Rat samples. It is validated for Western Blot, Simple Western, Intracellular Staining by Flow Cytometry, Immunocytochemistry, CyTOF-ready.

Specifications

Antigen ERK1/ERK2 (p Tyr187, p Tyr204, p Thr185, p Thr202)
Applications Western Blot, Flow Cytometry, Immunocytochemistry, CyTOF
Classification Monoclonal
Clone 269434
Concentration LYOPH
Conjugate Unconjugated
Dilution Western Blot 0.5 ug/mL, Simple Western 5 ug/mL, Intracellular Staining by Flow Cytometry 2.5 ug/10^6 cells, Immunocytochemistry 8-25 ug/mL, CyTOF-ready
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS. with No Preservative
Host Species Rabbit
Immunogen Phosphopeptide containing ERK1 T202/Y204 site
Purification Method Protein A or G purified from hybridoma culture supernatant
Quantity 25 μg
Regulatory Status RUO
Primary or Secondary Primary
Test Specificity Detects human, mouse, and rat ERK1 and ERK2 dually phosphorylated at T202/Y204 or T185/Y187, respectively.
Reconstitution Reconstitute at 0.5 mg/mL in sterile PBS.
Target Species Human, Mouse, Rat
Content And Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Form Purified
Isotype IgG
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