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Human/Mouse Sonic Hedgehog N-Terminus Antibody, R&D Systems™
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Catalog No. MAB464
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500 μg
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Catalog No. Quantity
MAB464 500 μg
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Catalog No. MAB464 Supplier R&D Systems Supplier No. MAB464
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Description

Rat Monoclonal Antibody has been used in 3 publications

Sonic Hedgehog/Shh Monoclonal specifically detects Sonic Hedgehog/Shh in Human, Mouse samples. It is validated for Western Blot.

Specifications

Antigen Sonic Hedgehog/Shh
Applications Western Blot
Classification Monoclonal
Clone 171001
Conjugate Unconjugated
Dilution Western Blot 2 ug/mL
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS. with No Preservative
Gene Accession No. Q62226
Gene Alias HHG1, HHG-1, HLP3, HPE3, MCOPCB5, MCOPCB5sonic hedgehog (Drosophila) homolog, Shh, ShhNC, SMMCI, SMMCIsonic hedgehog homolog (Drosophila), sonic hedgehog, sonic hedgehog homolog, sonic hedgehog protein, TPT, TPTPS
Gene Symbols SHH
Host Species Rat
Immunogen E. coli-derived recombinant mouse Sonic Hedgehog Cys25-Gly198 (Lys122Arg) Accession # Q62226
Purification Method Protein A or G purified from hybridoma culture supernatant
Quantity 500 μg
Regulatory Status RUO
Primary or Secondary Primary
Gene ID (Entrez) 6469
Test Specificity Detects the N-terminal peptide of human (amino acids 24-197) and mouse (amino acids 25-198) Shh in direct ELISAs and Western blots. Shows 50-100% cross-reactivity with the N-terminal peptides of recombinant mouse (rm) Dhh (amino acids 23-198) and rmIhh (amino acids 66-240) and no cross-reactivity with the C-terminal peptides from rmShh (amino acids 199-437), rmDhh (amino acids 199-396), or rmIhh (amino acids 241-449).
Reconstitution Reconstitute at 0.5 mg/mL in sterile PBS.
Target Species Human, Mouse
Content And Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Form Purified
Isotype IgG2a
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