Human/Mouse Sonic Hedgehog N-Terminus Antibody, R&D Systems™

Rat Monoclonal Antibody has been used in 3 publications

Supplier:  R&D Systems MAB464

Catalog No. MAB464

Description

Sonic Hedgehog/Shh Monoclonal specifically detects Sonic Hedgehog/Shh in Human, Mouse samples. It is validated for Western Blot.

Specifications

• Antigen: Sonic Hedgehog/Shh
• Classification: Monoclonal
• Conjugate: Unconjugated
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS. with No Preservative
• Gene Alias: HHG1, HHG-1, HLP3, HPE3, MCOPCB5, MCOPCB5sonic hedgehog (Drosophila) homolog, Shh, ShhNC, SMMCI, SMMCIsonic hedgehog homolog (Drosophila), sonic hedgehog, sonic hedgehog homolog, sonic hedgehog protein, TPT, TPTPS
• Host Species: Rat
• Purification Method: Protein A or G purified from hybridoma culture supernatant
• Regulatory Status: RUO
• Gene ID (Entrez): 6469
• Reconstitution: Reconstitute at 0.5 mg/mL in sterile PBS.
• Content And Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Isotype: IgG2a

• Applications: Western Blot
• Clone: 171001
• Dilution: Western Blot 2 ug/mL
• Gene Accession No.: Q62226
• Gene Symbols: SHH
• Immunogen: E. coli-derived recombinant mouse Sonic Hedgehog Cys25-Gly198 (Lys122Arg) Accession # Q62226
• Quantity: 500 μg
• Primary or Secondary: Primary
• Test Specificity: Detects the N-terminal peptide of human (amino acids 24-197) and mouse (amino acids 25-198) Shh in direct ELISAs and Western blots. Shows 50-100% cross-reactivity with the N-terminal peptides of recombinant mouse (rm) Dhh (amino acids 23-198) and rmIhh (amino acids 66-240) and no cross-reactivity with the C-terminal peptides from rmShh (amino acids 199-437), rmDhh (amino acids 199-396), or rmIhh (amino acids 241-449).
• Target Species: Human, Mouse
• Form: Purified