Human/Mouse TNF-alpha Antibody, R&D Systems™

Goat Polyclonal Antibody has been used in 74 publications

Supplier:  R&D Systems AF410NA

Catalog No. AF410NA

Description

TNF-alpha Polyclonal specifically detects TNF-alpha in Human, Mouse samples. It is validated for Western Blot, Immunohistochemistry, Neutralization, Intracellular Staining by Flow Cytometry, Immunocytochemistry, CyTOF-ready, ELISA Capture (Matched Antibody Pair).

Specifications

• Antigen: TNF-alpha
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene Accession No.: P06804
• Gene Symbols: TNF
• Immunogen: E. coli-derived recombinant mouse TNF-alpha (R&D Systems, Catalog # 410-MT) Leu80-Leu235 Accession # P06804
• Quantity: 100 μg
• Primary or Secondary: Primary
• Test Specificity: Detects mouse TNF-alpha in ELISAs and Western blots. In sandwich immunoassays, approximately 50% cross-reactivity with recombinant rat TNF-alpha is observed and less than 1% cross-reactivity with recombinant human TNF-alpha is observed.
• Target Species: Mouse
• Isotype: IgG

• Applications: Western Blot, Neutralization, Flow Cytometry, Immunocytochemistry, CyTOF
• Concentration: LYOPH
• Dilution: Western Blot 0.1 ug/mL, Neutralization 0.1-0.4 ug/mL, Intracellular Staining by Flow Cytometry 2.5 ug/10^6 cells, Immunocytochemistry 5-15 ug/mL, CyTOF-ready, ELISA Capture (Matched Antibody Pair) 0.2-0.8 ug/mL
• Gene Alias: APC1 protein, Cachectin, Cachetin, DIF, TNF, TNF, monocyte-derived, TNF-a, TNFA, TNFalpha, TNF-alphacachectin, TNFATNF, macrophage-derived, TNFG1F, TNFSF1A, TNFSF2, TNFSF2TNF superfamily, member 2, tumor necrosis factor, tumor necrosis factor (TNF superfamily, member 2), tumor necrosis factor alpha, Tumor necrosis factor ligand superfamily member 2, tumor necrosis factor-alpha
• Host Species: Goat
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Gene ID (Entrez): 7124
• Reconstitution: Reconstitute at 0.2 mg/mL in sterile PBS.
• Content And Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.