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Human Notch-1 Intracellular Domain Antibody, R&D Systems™
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Catalog No. AF3647
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100 μg
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AF3647 100 μg
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Description

Sheep Polyclonal Antibody has been used in 3 publications

Notch-1 Polyclonal specifically detects Notch-1 in Human samples. It is validated for Western Blot, Flow Cytometry, Chromatin Immunoprecipitation (ChIP), Immunocytochemistry, CyTOF-ready.

Specifications

Antigen Notch-1
Applications ChIP Assay, CyTOF, Flow Cytometry, Immunocytochemistry
Classification Polyclonal
Concentration LYOPH
Conjugate Unconjugated
Dilution Western Blot 1 ug/mL, Flow Cytometry 2.5 ug/10^6 cells, Chromatin Immunoprecipitation (ChIP) 5 ug/5 x 10^6 cells, Immunocytochemistry 5-15 ug/mL, CyTOF-ready
Gene Accession No. P46531
Gene Alias EC 2.1.2.11, EC 3.4.21.68, hN1, Notch (Drosophila) homolog 1 (translocation-associated), notch 1Notch homolog 1, translocation-associated (Drosophila), Notch homolog 1, translocation-associated, Notch1, TAN1, TAN1neurogenic locus notch homolog protein 1, Translocation-associated notch protein TAN-1
Gene Symbols NOTCH1
Host Species Sheep
Immunogen E. coli-derived recombinant human Notch-1 Gly2428-Lys2556 Accession # P46531
Purification Method Affinity Purified
Quantity 100 μg
Regulatory Status RUO
Primary or Secondary Primary
Gene ID (Entrez) 4851
Test Specificity Detects the intracellular domain (ICD) of human Notch-1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human (rh) Notch-2 ICD, rhNotch-3 ICD, and rhNotch-4 ICD is observed.
Reconstitution Reconstitute at 0.2 mg/mL in sterile PBS.
Target Species Human
Content And Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Isotype IgG
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