Human Notch-1 Intracellular Domain Antibody, R&D Systems™

Sheep Polyclonal Antibody has been used in 3 publications

Supplier:  R&D Systems AF3647

Catalog No. AF3647

Description

Notch-1 Polyclonal specifically detects Notch-1 in Human samples. It is validated for Western Blot, Flow Cytometry, Chromatin Immunoprecipitation (ChIP), Immunocytochemistry, CyTOF-ready.

Specifications

• Antigen: Notch-1
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene Accession No.: P46531
• Gene Symbols: NOTCH1
• Immunogen: E. coli-derived recombinant human Notch-1 Gly2428-Lys2556 Accession # P46531
• Quantity: 100 μg
• Primary or Secondary: Primary
• Test Specificity: Detects the intracellular domain (ICD) of human Notch-1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human (rh) Notch-2 ICD, rhNotch-3 ICD, and rhNotch-4 ICD is observed.
• Target Species: Human
• Isotype: IgG

• Applications: ChIP Assay, CyTOF, Flow Cytometry, Immunocytochemistry
• Concentration: LYOPH
• Dilution: Western Blot 1 ug/mL, Flow Cytometry 2.5 ug/10^6 cells, Chromatin Immunoprecipitation (ChIP) 5 ug/5 x 10^6 cells, Immunocytochemistry 5-15 ug/mL, CyTOF-ready
• Gene Alias: EC 2.1.2.11, EC 3.4.21.68, hN1, Notch (Drosophila) homolog 1 (translocation-associated), notch 1Notch homolog 1, translocation-associated (Drosophila), Notch homolog 1, translocation-associated, Notch1, TAN1, TAN1neurogenic locus notch homolog protein 1, Translocation-associated notch protein TAN-1
• Host Species: Sheep
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Gene ID (Entrez): 4851
• Reconstitution: Reconstitute at 0.2 mg/mL in sterile PBS.
• Content And Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.