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R&D Systems™ Recombinant Human PD-1 Fc Chimera Protein, CF

Extensive quality control produces lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Binding Activity

Manufacturer:  R&D Systems™ 1086PD01M

Catalog No. 10-86P-D01M

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Measured by its binding ability in a functional ELISA. When Recombinant Human PD-1 Fc Chimera is immobilized at 0.1 μg/mL (100 μL/well), Recombinant Human B7-H1/PD-L1 Fc Chimera (Catalog # 156-B7 ) binds with a typical ED50 of 0.15-0.75 μg/mL.

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Ships at ambient temperature.



Binding Activity
CD279, CD279 antigen, hPD-1, PD1, PD1hPD-l, PDCD1, programmed cell death 1, programmed cell death protein 1, Protein PD-1, SLEB2
M.W. (Observed): 60-70 kDa, reducing conditions; M.W. (theoretical): 42.6 kDa (monomer)
1 mg
Reconstitute at 0.5 mg/mL in sterile PBS.
Mouse myeloma cell line, NS0-derived human PD-1 protein Human PD-1 (Leu25-Gln167) (N-terminus) Accession # Q15116 IEGRMD Human IgG1 (Pro100-Lys330) (C-terminus)
0.15 to 0.75μg/mL
<0.01 EU / 1 µg of the protein by the LAL method.
Lyophilized from a 0.2 µm filtered solution in PBS.
Recombinant protein
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
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