Invitrogen™ Human PD-1 His-tag Fc Chimera Recombinant Protein

Description

Recombinant Protein

Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/mL or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by cognate peptides bound to MHC molecules on antig en-presenting cells (APC). T-cell activation is generally self-limited as activated T cells express receptors such as PD-1 (also known as PDCD-1) that mediate inhibitory signals from the APC. PD-1 can bind two different but related ligands, PDL-1 and PDL-2. Upon binding to either of these ligands, signals generated by PD-1 inhibit the activation of the immune response in the absence of 'danger signals' such as LPS or other molecules associated with bacteria or other pathogens. Evidence for this is seen in PD1-null mice who exhibit hyperactivated immune systems and autoimmune diseases. Despite its predicted molecular weight, PD-1 often migrates at higher molecular weight in SDS-PAGE.

Specifications

• Accession Number: Q15116
• For Use With (Application): ELISA, Immunohistochemistry, Western Blot
• Formulation: PBS with 0.01% Tween 80, 5% mannitol, 5% trehalose and no preservative; pH 7.4
• Gene ID (Entrez): 5133
• Molecular Weight (g/mol): 44 kDa
• Name: Human PD-1 His-tag Fc Chimera
• Quantity: 50 μg
• Source: HEK293 cells
• Regulatory Status: RUO
• Endotoxin Concentration: <1.0 EU/ μg
• Gene Alias: CD279; EGK_05005; hPD1; hPD-1; hPD-l; hSLE1; Ly101; mPD-1; PD1; PD-1; Pdc1; Pdcd1; programmed cell death 1; programmed cell death 1 protein; programmed cell death protein 1; programmed cell death protein 1-like; programmed death 1; Protein PD1; protein PD-1; sCD279; SLEB2; soluble CD279; systemic lupus erythematosus susceptibility 2
• Common Name: PD-1 (CD279)
• Gene Symbol: Pdcd1
• Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized recombinant human PD-L2 (at 1 μg/mL (100 μL/well) can bind human PD1/Fc chimera with a linear range of 7.8-1000 ng/mL)
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Protein Tag: Fc-tag, His-tag
• Sequence: Human PD1, amino acids Met1-Gln167 (Accession # NP_005009.2) with a C-terminal His-tagged human IgG1 Fc-tag
• Content And Storage: -20°Cor to +80°C if preferred
• Expression System: HEK293 cells
• Form: Lyophilized
• Purity or Quality Grade: >97% by SDS-PAGE