Invitrogen™ PDGF-BB Human ELISA Kit 

For the quantitative detection of human PDGF-BB.

Manufacturer:  Invitrogen™ BMS2071TEN

Catalog No. 50-246-660

Includes: Aluminium pouch(es) with a Microwell Plate coated with antibody to human PDGF-BB
Biotin-Conjugate anti-human PDGF-BB antibody
Streptavidin-HRP
Human PDGF-BB Standard lyophilized, 4ng/mL upon reconstitution
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Blue-Dye
Green-Dye
Red-Dye
Adhesive Films

Description

The protein encoded by this gene is a member of the platelet-derived growth factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a motif of eight cysteines. This gene product can exist either as a homodimer or as a heterodimer with the platelet-derived growth factor alpha polypeptide , where the dimers are connected by disulfide bonds. Mutations in this gene are associated with meningioma. Reciprocal translocations between chromosomes 22 and 7, at sites where this gene and that for COL1A1 are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans resulting from unregulated expression of growth factor. Two alternatively spliced transcript variants encoding different isoforms have been identified for this gene.

Specifications

• Accession Number: P01127
• Conjugate: Biotin
• Gene ID (Entrez): 5155
• Product Type: ELISA Kit
• Sensitivity: 4.6 pg/mL
• Sample Volume: Plasma, 20 μL|Serum, 20 μL|Supernatant, 20 μL
• Test Time: 1 hr. 20 min.
• Target: Human
• Synonym: Platelet derived growth factor BB
• Intraassay CV: 4%
• Target Species: Human
• Label or Dye: HRP

• Assay Range: 31.3-2,000 pg/mL
• For Use With (Application): ELISA
• Kit Contents: Adhesive Plate Covers
• Regulatory Status: RUO
• Sample Size: 50 μL (1:10 prediluted)
• Storage Requirements: 2°C to 8°C
• Total Assay Time: 3 hr. 30 min.
• Gene Symbols: IBGC5,PDGF-2,PDGF2,SIS,SSV,c-sis
• Interassay CV: 8.4%
• Quantity: 10 x 96 Tests
• Sample Type: Plasma, Serum, Supernatant
• For Use With (Equipment): Colorimetric Microplate Reader

For Research Use Only