Abnova™ Human PTK2 (NP_722560.1, 1 a.a. - 527 a.a.) Full-length Recombinant Protein with GST-tag co-expressed with His-tagged WNK1 (NP_061852.1, 1 a.a. - 491 a.a.) expressed in Baculovirus infected Sf21 cells

Description

Used for Func, SDS-PAGE

This gene encodes a cytoplasmic protein tyrosine kinase which is found concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Activation of this gene may be an important early step in cell growth and intracellular signal transduction pathways triggered in response to certain neural peptides or to cell interactions with the extracellular matrix. At least four transcript variants encoding four different isoforms have been found for this gene, but the full-length natures of only two of them have been determined. [provided by RefSeq]

Specifications

• Accession Number: NP_722560.1
• For Use With (Application): Functional Study, SDS-PAGE
• Formulation: Liquid
• Gene ID (Entrez): 5747
• Molecular Weight (g/mol): 103kDa
• Name: PTK2 (Human) Recombinant Protein
• Preparation Method: Baculovirus infected insect cell (Sf21) expression system
• Purification Method: Glutathione sepharose chromatography
• Quality Control Testing: Loading 1 ug protein in SDS-PAGE
• Quantity: 5 μg
• Storage Requirements: Store at -80°C. Aliquot to avoid repeated freezing and thawing.
• Regulatory Status: RUO
• Gene Alias: FADK/FAK/FAK1/pp125FAK
• Common Name: PTK2
• Gene Symbol: PTK2
• Biological Activity: The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorescence-labeled substrate and Mg (or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip™3000.
  Substrate : Blk/Lyntide. ATP: 100 μM.
• Species: Baculovirus
• Recombinant: Recombinant
• Protein Tag: GST
• Expression System: Baculovirus infected insect cell (Sf21) expression system
• Form: Liquid
• Purity or Quality Grade: 62% by SDS-PAGE/CBB staining.