Human PU.1/Spi-1 Antibody, R&D Systems™

Mouse Monoclonal Antibody

Supplier:  R&D Systems MAB5870SP

Catalog No. MAB5870SP

Description

PU.1/Spi-1 Monoclonal specifically detects PU.1/Spi-1 in Human samples. It is validated for Western Blot, Intracellular Staining by Flow Cytometry, CyTOF-ready.

Specifications

• Antigen: PU.1/Spi-1
• Classification: Monoclonal
• Concentration: LYOPH
• Dilution: Western Blot 2 ug/mL, Intracellular Staining by Flow Cytometry 2.5 ug/10^6 cells, CyTOF-ready
• Gene Accession No.: NP_001074016
• Gene Symbols: SPI1
• Immunogen: E. coli-derived recombinant human PU.1/Spi-1 Met1-Lys169 Accession # NP_001074016
• Quantity: 25 μg
• Primary or Secondary: Primary
• Test Specificity: Detects human PU.1/Spi-1 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) Spi-B, recombinant mouse (rm) PU.1/Spi-1, or rmSpi-B is observed. In Western blots, approximately 25% cross-reactivity with rhSpi-B and rmPU.1/Spi-1 is observed.
• Target Species: Human
• Form: Purified

• Applications: Western Blot, Flow Cytometry, CyTOF
• Clone: 732322
• Conjugate: Unconjugated
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS. with No Preservative
• Gene Alias: hematopoietic transcription factor PU.1, OF, PU.1, SFPI1, SFPI131 kDa transforming protein, SPI1, SPI-1, SPI-1 proto-oncogene, SPI-A, spleen focus forming virus (SFFV) proviral integration oncogene spi1,31 kDa-transforming protein, transcription factor PU.1
• Host Species: Mouse
• Purification Method: Protein A or G purified from hybridoma culture supernatant
• Regulatory Status: RUO
• Gene ID (Entrez): 6688
• Reconstitution: Sterile PBS to a final concentration of 0.5 mg/mL.
• Content And Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Isotype: IgG2b

For Research Use Only