Human PU.1/Spi-1 Antibody, R&D Systems™

Sheep Polyclonal Antibody

Supplier:  R&D Systems AF5870

Catalog No. AF5870

Description

PU.1/Spi-1 Polyclonal specifically detects PU.1/Spi-1 in Human samples. It is validated for Western Blot, Intracellular Staining by Flow Cytometry, Immunocytochemistry, CyTOF-ready.

Specifications

• Antigen: PU.1/Spi-1
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene Accession No.: NP_001074016
• Gene Symbols: SPI1
• Immunogen: E. coli-derived recombinant human PU.1 Met1-Lys169 Accession # NP_001074016
• Quantity: 100 μg
• Primary or Secondary: Primary
• Test Specificity: Detects human PU.1 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human SPI-B is observed.
• Target Species: Human
• Isotype: IgG

• Applications: Western Blot, Flow Cytometry, Immunocytochemistry, CyTOF
• Concentration: LYOPH
• Dilution: Western Blot 1 ug/mL, Intracellular Staining by Flow Cytometry 2.5 ug/10^6 cells, Immunocytochemistry 5-15 ug/mL, CyTOF-ready
• Gene Alias: hematopoietic transcription factor PU.1, OF, PU.1, SFPI1, SFPI131 kDa transforming protein, SPI1, SPI-1, SPI-1 proto-oncogene, SPI-A, spleen focus forming virus (SFFV) proviral integration oncogene spi1,31 kDa-transforming protein, transcription factor PU.1
• Host Species: Sheep
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Gene ID (Entrez): 6688
• Reconstitution: Reconstitute at 0.2 mg/mL in sterile PBS.
• Content And Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.